Yang Dongzhi, Chen Qifan, Wang Wenxing, Xu Shukun
Department of Chemistry, Northeastern University, Shenyang 110004, People's Republic of China.
Luminescence. 2008 May-Jun;23(3):169-74. doi: 10.1002/bio.1029.
With excellent optical properties, quantum dots (QDs) have been made as attractive molecular probes for labelling cells in biological research. In this study high-quality CdSe QDs prepared in a paraffin-oleic acid system were used as fluorescent labels in direct and indirect detection of carcinoembryonic antigen (CEA), a cancer marker expressed on the surface of HeLa cells. The primary antibody (Ab) (rabbit anti-CEA8) and secondary Ab (goat anti-rabbit IgG) were covalently linked to carboxyl-functioned CdSe QDs, and both the QDs-antibody and QDs-IgG probes were successfully used to label HeLa cells. The present study demonstrates the practicability of CdSe QDs as an attractive type of fluorescent labels for biological applications such as protein probes and cell imaging.
量子点（QDs）具有出色的光学特性，已成为生物研究中用于标记细胞的有吸引力的分子探针。在本研究中，在石蜡-油酸体系中制备的高质量CdSe量子点被用作荧光标记物，用于直接和间接检测癌胚抗原（CEA），这是一种在HeLa细胞表面表达的癌症标志物。将一抗（Ab）（兔抗CEA8）和二抗（山羊抗兔IgG）共价连接到具有羧基功能的CdSe量子点上，量子点-抗体和量子点-IgG探针均成功用于标记HeLa细胞。本研究证明了CdSe量子点作为一种有吸引力的荧光标记物在蛋白质探针和细胞成像等生物应用中的实用性。
