Beliaeva T N, Salova A V, Leont'eva E A, Mozhenok T P, Kornilova E S, Krolenko S A
Tsitologiia. 2009;51(10):830-7.
The problem of non-specific binding of quantum dots (QDs) with cells is very important but not fully understood taking into account the possible application of QDs in medical and fundamental studies. The interactions of untargeted CdSe/ZnS QDs with isolated frog muscle fibres, HeLa cells and J774 cells were investigated. The observations were performed on living cells using laser confocal microscopy (Leica TCS SL). The QDs covered with polyethylenglycol without any functional reactive groups with emission maximum at 565 nm were used in the study. This type of QDs is suggested to prevent an interaction of QDs with biological molecules. It has been shown that QDs do not enter HeLa cells, T-system and sarcoplasm of skeletal muscle fibres. However, during long-term incubation J774 cells can uptake QDs. The data obtained has demonstrated the diversity of interactions of untargeted QDs with different cell types and are important for understanding of the problems of non-selective uptake and cytotoxicity of QDs.
考虑到量子点在医学和基础研究中的潜在应用，量子点（QDs）与细胞的非特异性结合问题非常重要，但尚未完全了解。研究了未靶向的CdSe/ZnS量子点与分离的青蛙肌肉纤维、HeLa细胞和J774细胞的相互作用。使用激光共聚焦显微镜（徕卡TCS SL）对活细胞进行观察。本研究使用了覆盖有聚乙二醇且无任何功能性反应基团、发射最大值在565nm的量子点。建议使用这种类型的量子点来防止量子点与生物分子相互作用。结果表明，量子点不会进入HeLa细胞、骨骼肌纤维的T系统和肌浆。然而，在长期孵育过程中，J774细胞可以摄取量子点。所获得的数据证明了未靶向量子点与不同细胞类型相互作用的多样性，对于理解量子点的非选择性摄取和细胞毒性问题具有重要意义。
