在基因工程大肠杆菌中合成和表征 CdS 量子点。
Biosynthesis and characterization of CdS quantum dots in genetically engineered Escherichia coli.
机构信息
Department of Chemistry, Northeastern University, Shenyang 110819, PR China.
出版信息
J Biotechnol. 2011 May 20;153(3-4):125-32. doi: 10.1016/j.jbiotec.2011.03.014. Epub 2011 Mar 31.
Abstract
Quantum dots (QDs) were prepared in genetically engineered Escherichia coli (E. coli) through the introduction of foreign genes encoding a CdS binding peptide. The CdS QDs were successfully separated from the bacteria through two methods, lysis and freezing-thawing of cells, and purified with an anion-exchange resin. High-resolution transmission electron microscopy, X-ray diffraction, luminescence spectroscopy, and energy dispersive X-ray spectroscopy were applied to characterize the as-prepared CdS QDs. The effects of reactant concentrations, bacteria incubation times, and reaction times on QD growth were systematically investigated. Our work demonstrates that genetically engineered bacteria can be used to synthesize QDs. The biologically synthesized QDs are expected to be more biocompatible probes in bio-labeling and imaging.
摘要
量子点 (QDs) 通过引入编码 CdS 结合肽的外源基因，在基因工程大肠杆菌 (E. coli) 中制备。通过细胞裂解和冻融两种方法成功地将 CdS QDs 从细菌中分离出来，并通过阴离子交换树脂进行纯化。高分辨率透射电子显微镜、X 射线衍射、发光光谱和能量色散 X 射线光谱用于表征所制备的 CdS QDs。系统研究了反应物浓度、细菌孵育时间和反应时间对 QD 生长的影响。我们的工作表明，基因工程细菌可用于合成 QDs。生物合成的 QDs 有望成为生物标记和成像中更具生物相容性的探针。
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