Slobedman Barry, Cheung Allen K L
Centre for Virus Research, Westmead Millennium Institute, Westmead, New South Wales, Australia.
Methods Mol Med. 2008;141:153-75. doi: 10.1007/978-1-60327-148-6_9.
Human cytomegalovirus (HCMV) is one of the largest known DNA viruses. It is ubiquitous, and following resolution of primary productive infection, it persists in the human host by establishing a lifelong latent infection in myeloid lineage cells such as monocytes and their progenitors. Most adults with HCMV infection are healthy but it can cause neurologic deficits in infants, and remains an important cause of morbidity and mortality in the immunosuppressed patient. Microarray-based studies of HCMV have provided useful information about genes that are transcriptionally active during both productive and latent phases of infection. This chapter describes how to study genes in HCMV using microarrays and two cell types (productively infected human foreskin fibroblasts, and latently infected primary human myeloid progenitor cells).
人巨细胞病毒（HCMV）是已知最大的DNA病毒之一。它广泛存在，在原发性生产性感染消退后，通过在单核细胞及其祖细胞等髓系细胞中建立终身潜伏感染而在人类宿主中持续存在。大多数感染HCMV的成年人是健康的，但它可导致婴儿神经功能缺损，并且仍然是免疫抑制患者发病和死亡的重要原因。基于微阵列的HCMV研究提供了关于在感染的生产性和潜伏阶段转录活跃的基因的有用信息。本章描述了如何使用微阵列和两种细胞类型（生产性感染的人包皮成纤维细胞和潜伏感染的原代人髓系祖细胞）来研究HCMV中的基因。
