Kim Guk Bae, Yoon Yae Jin, Shin Tae Joo, Youn Hwa Shik, Gho Yong Song, Lee Sang Joon
System Bio-Dynamics National Core Research Center, Pohang University of Science and Technology, Republic of Korea.
Microsc Res Tech. 2008 Sep;71(9):639-43. doi: 10.1002/jemt.20601.
In this study, we visualized the internal structures of various bio-samples and found the optimum conditions of test samples for the 7 keV hard X-ray microscope of the Pohang light source. From the captured X-ray images, we could observe the intercellular and intracellular structures of dehydrated human cells and mouse tumor tissues without using any staining materials in a spatial resolution better than 100 nm. The metastasized lung tissue, which was several tens of micrometers in thickness, was found to be very well suited to this hard X-ray microscope system, because it is nearly impossible to observe such a nontransparent and thick sample with a high spatial resolution better than 100 nm using any microscopes such as a soft X-ray microscope, an optical microscope, or an electron microscope.
在本研究中，我们对各种生物样本的内部结构进行了可视化，并找到了浦项光源7keV硬X射线显微镜测试样本的最佳条件。从捕获的X射线图像中，我们可以在不使用任何染色材料的情况下，以优于100nm的空间分辨率观察脱水人类细胞和小鼠肿瘤组织的细胞间和细胞内结构。发现厚度为几十微米的转移肺组织非常适合这种硬X射线显微镜系统，因为使用软X射线显微镜、光学显微镜或电子显微镜等任何显微镜，几乎不可能以优于100nm的高空间分辨率观察这样不透明且厚的样本。
