Department of Physics and Photon Science & School of Materials Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, 61005, Korea.
European XFEL, Schenefeld, 22869, Germany.
Sci Rep. 2017 May 12;7(1):1850. doi: 10.1038/s41598-017-01833-x.
We report a three dimensional (3D) quantitative visualization of a mammalian mitochondrion by coherent x-ray diffractive imaging (CXDI) using synchrotron radiation. The internal structures of a mitochondrion from a mouse embryonic fibroblast cell line (NIH3T3) were visualized by tomographic imaging at approximately 60 nm resolution without the need for sectioning or staining. The overall structure consisted of a high electron density region, composed of the outer and inner membranes and the cristae cluster, which enclosed the lower density mitochondrial matrix. The average mass density of the mitochondrion was about 1.36 g/cm. Sectioned images of the cristae reveal that they have neither a baffle nor septa shape but were instead irregular. In addition, a high resolution, about 14 nm, 2D projection image was captured of a similar mitochondrion with the aid of strongly scattering Au reference objects. Obtaining 3D images at this improved resolution will allow CXDI to be an effective and nondestructive method for investigating the innate structure of mitochondria and other important life supporting organelles.
我们通过使用同步辐射的相干 X 射线衍射成像（CXDI）报告了哺乳动物线粒体的三维（3D）定量可视化。通过层析成像，在无需切片或染色的情况下，以约 60nm 的分辨率对来自小鼠胚胎成纤维细胞系（NIH3T3）的线粒体的内部结构进行了可视化。整体结构由一个高电子密度区域组成，该区域由外膜、内膜和嵴簇组成，这些结构包围着密度较低的线粒体基质。线粒体的平均质量密度约为 1.36g/cm³。对嵴的切片图像表明，它们既没有隔板也没有隔片形状，而是不规则的。此外，借助强烈散射的 Au 参考物体，还捕获了类似线粒体的高分辨率（约 14nm）2D 投影图像。以这种提高的分辨率获得 3D 图像将使 CXDI 成为一种有效且无损的方法，用于研究线粒体和其他重要生命支持细胞器的固有结构。
