诱导龈上菌斑中Toll样受体（TLR）2和TLR4介导刺激的活性分析。

Analysis of the activity to induce toll-like receptor (TLR)2- and TLR4-mediated stimulation of supragingival plaque.

作者信息

Yoshioka Hidenobu, Yoshimura Atsutoshi, Kaneko Takashi, Golenbock Douglas T, Hara Yoshitaka

机构信息

Department of Periodontology, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki, Japan.

出版信息

J Periodontol. 2008 May;79(5):920-8. doi: 10.1902/jop.2008.070516.

DOI:10.1902/jop.2008.070516

PMID:18454672

Abstract

BACKGROUND

The deleterious effects of the accumulation of supragingival plaque are well known, but the role of the proinflammatory property of supragingival plaque in periodontal diseases has not been completely elucidated. The aim of this study was to determine the relevance of Toll-like receptor (TLR)2- and TLR4-stimulating activity of supragingival plaque to periodontal parameters.

METHODS

We isolated 144 supragingival plaque samples and analyzed TLR2- and TLR4-stimulating activity using genetically engineered Chinese hamster ovary reporter cells that express a reporter molecule upon activation of nuclear factor-kappa B through TLR2 or TLR4. The numbers of Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans), and Streptococcus mutans cells in each plaque sample were determined by real-time polymerase chain reaction.

RESULTS

The activity to induce TLR4-mediated stimulation, but not TLR2-mediated stimulation, was positively associated with the plaque score and bleeding on probing score of the teeth from which the plaque samples were taken. The activity to induce TLR2-mediated stimulation, but not TLR4-mediated stimulation, was negatively associated with probing depth and clinical attachment level. The ratio of TLR4-/TLR2-mediated stimulation was positively associated with all of those parameters. The number of P. gingivalis cells in each plaque sample was associated with the plaque score and clinical attachment level, but no strong association was observed between the ratio of examined bacteria in each plaque sample and the activity to induce TLR2- or TLR4-mediated stimulation, except for a weak correlation between the ratio of A. actinomycetemcomitans cells and the activity to induce TLR4-mediated stimulation.

CONCLUSION

The TLR2- and TLR4-stimulating activity of supragingival plaque is associated with clinical parameters for gingivitis and periodontitis.

摘要

背景

龈上菌斑积聚的有害影响众所周知，但龈上菌斑的促炎特性在牙周疾病中的作用尚未完全阐明。本研究的目的是确定龈上菌斑的Toll样受体（TLR）2和TLR4刺激活性与牙周参数的相关性。

方法

我们分离了144份龈上菌斑样本，并使用基因工程中国仓鼠卵巢报告细胞分析TLR2和TLR4刺激活性，该细胞在通过TLR2或TLR4激活核因子-κB时表达报告分子。通过实时聚合酶链反应测定每个菌斑样本中牙龈卟啉单胞菌、伴放线聚集杆菌（以前称为伴放线放线杆菌）和变形链球菌细胞的数量。

结果

诱导TLR4介导刺激的活性而非TLR2介导刺激的活性与采集菌斑样本的牙齿的菌斑评分和探诊出血评分呈正相关。诱导TLR2介导刺激的活性而非TLR4介导刺激的活性与探诊深度和临床附着水平呈负相关。TLR4/TLR2介导刺激的比率与所有这些参数呈正相关。每个菌斑样本中牙龈卟啉单胞菌细胞的数量与菌斑评分和临床附着水平相关，但除了伴放线聚集杆菌细胞比率与诱导TLR4介导刺激的活性之间存在弱相关性外，未观察到每个菌斑样本中检测到的细菌比率与诱导TLR2或TLR4介导刺激的活性之间有强关联。