Kikkert R, Laine M L, Aarden L A, van Winkelhoff A J
Department of Immunopathology, Sanquin Research, Amsterdam, and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, The Netherlands.
Oral Microbiol Immunol. 2007 Jun;22(3):145-51. doi: 10.1111/j.1399-302X.2007.00335.x.
BACKGROUND/AIMS: Periodontitis is a chronic infectious disease associated with a gram-negative subgingival microflora. Bacterial components stimulate, among other receptors, Toll-like receptor (TLR) 2 and/or TLR4. Accumulating evidence indicates that both qualitatively and quantitatively distinct immune responses result from the triggering of TLR2 as compared to TLR4 triggering. The aim was to study the interaction of Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Tannerella forsythensis, Prevotella intermedia, Prevotella nigrescens, Fusobacterium nucleatum and Veillonella parvula with TLR2 and TLR4. We investigated all known serotypes (K(-), K1-K6) of P. gingivalis and A. actinomycetemcomitans serotype a-e strains for their potency to stimulate cytokine production.
Human embryonic kidney (HEK) cells, stably transfected with CD14, CD14-TLR2, or CD14-TLR4 and whole blood were stimulated with bacterial sonicates. Cytokine production (interleukin-6, -8, -10 and -12) was measured in the supernatant by enzyme-linked immunosorbent assay.
All test bacteria stimulated HEK-CD14-TLR2, but only A. actinomycetemcomitans and V. parvula stimulated HEK-CD14-TLR4. No differences were found in the activation of HEK-CD14-TLR2/4, or cytokine production in whole blood between serotypes of P. gingivalis and A. actinomycetemcomitans.
Gram-negative periodontal bacteria predominantly stimulated TLR2, which may be of importance for the Th1/Th2 cell orientation of the immune response in periodontitis.
背景/目的：牙周炎是一种与革兰氏阴性龈下微生物群相关的慢性感染性疾病。细菌成分除了刺激其他受体外，还会刺激Toll样受体（TLR）2和/或TLR4。越来越多的证据表明，与TLR4触发相比，TLR2触发会导致在质量和数量上都不同的免疫反应。目的是研究牙龈卟啉单胞菌、伴放线放线杆菌、福赛坦氏菌、中间普氏菌、变黑普氏菌、具核梭杆菌和小韦荣球菌与TLR2和TLR4的相互作用。我们研究了牙龈卟啉单胞菌的所有已知血清型（K(-)、K1-K6）以及伴放线放线杆菌血清型a-e菌株刺激细胞因子产生的能力。
用细菌超声裂解物刺激稳定转染了CD14、CD14-TLR2或CD14-TLR4的人胚肾（HEK）细胞和全血。通过酶联免疫吸附测定法测量上清液中的细胞因子产生（白细胞介素-6、-8、-10和-12）。
所有测试细菌均刺激HEK-CD14-TLR2，但只有伴放线放线杆菌和小韦荣球菌刺激HEK-CD14-TLR4。牙龈卟啉单胞菌和伴放线放线杆菌各血清型之间在HEK-CD14-TLR2/4的激活或全血中的细胞因子产生方面未发现差异。
革兰氏阴性牙周细菌主要刺激TLR2，这可能对牙周炎免疫反应中Th1/Th2细胞方向具有重要意义。
