Salih V, Patel A, Knowles J C
Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, 256 Gray's Inn Road, London, WC1X 8LD, UK.
Biomed Mater. 2007 Mar;2(1):11-20. doi: 10.1088/1748-6041/2/1/003. Epub 2007 Jan 12.
It has been shown that the addition of zinc to hydroxyapatite promotes osteoblast cell adhesion (Ishaug et al 1994 J. Biomed. Mater. Res. A 28 1445-53). Therefore, this study presents a series of phosphate-based glass compositions that contain varying amounts of zinc in order to promote osteoblast cell adhesion. The compositions investigated were P(50)Ca((40-x))Na(10) where x = 0, 1, 2, 3, 4 and 5 mol%. The dissolution rate and effect on pH of glass discs were investigated and ion release from the glass discs was examined in distilled water at 37 degrees C after 1, 2, 3, 4 and 7 days. The attachment of osteoblast-like cells (HOB) was observed by SEM on the glass discs. A linear decrease in the %mass of the glass discs was observed for all compositions for the duration of the study. The dissolution rates were similar for all the compositions. After 7 days, a mass loss of 3-6% was observed for all the compositions. The pH of distilled water decreased to a range of pH 4.5-5.5 from pH 7 after 1 day, and remained in this range for the duration of the study. The composition containing 0 mol% Zn reduced the pH to a lesser extent than the composition containing the highest amount of Zn. Furthermore, Zn(2+) ion release was observed from all the Zn-containing compositions. These glass compositions may therefore be suitable for tissue engineering applications.
研究表明，向羟基磷灰石中添加锌可促进成骨细胞的黏附（Ishaug等人，1994年，《生物医学材料研究杂志A》28卷，1445 - 1453页）。因此，本研究提出了一系列含不同锌含量的磷酸盐基玻璃组合物，以促进成骨细胞的黏附。所研究的组合物为P(50)Ca((40 - x))Na(10)，其中x = 0、1、2、3、4和5摩尔%。研究了玻璃圆盘的溶解速率及其对pH值的影响，并在37℃下于蒸馏水中分别放置1、2、3、4和7天后，检测了玻璃圆盘的离子释放情况。通过扫描电子显微镜观察了类成骨细胞（HOB）在玻璃圆盘上的附着情况。在整个研究期间，观察到所有组合物的玻璃圆盘质量百分比呈线性下降。所有组合物的溶解速率相似。7天后，所有组合物的质量损失均为3 - 6%。蒸馏水中的pH值在1天后从pH 7降至pH 4.5 - 5.5的范围，并在整个研究期间保持在该范围内。含0摩尔%锌的组合物使pH值降低的程度小于含锌量最高的组合物。此外，从所有含锌组合物中均观察到Zn(2+)离子的释放。因此，这些玻璃组合物可能适用于组织工程应用。
