Bil M, Ryszkowska J, Roether J A, Bretcanu O, Boccaccini A R
Faculty of Materials Science and Engineering, Warsaw University of Technology, Woloska141, 02-507 Warsaw, Poland.
Biomed Mater. 2007 Jun;2(2):93-101. doi: 10.1088/1748-6041/2/2/006. Epub 2007 Mar 14.
Polyurethane (PUR) and polyurethane/poly(d, l-lactide) acid (PUR/PDLLA) based scaffolds coated with Bioglass particles for application in bone tissue engineering were fabricated. The slurry-dipping method was used for coating preparation. The homogeneous structure of the Bioglass coatings on the surface of the PUR and PUR/PDLLA foams indicated a good adhesion of the bioactive glass particles to polyurethane without any additional surface treatment. In vitro studies in simulated body fluid (SBF) were performed to study the influence of Bioglass coating on biodegrability and bioactivity of PUR-based scaffolds. The surface of Bioglass-coated samples was covered by a layer of carbonate-containing apatite after 7 days of immersion in SBF, while in uncoated polymer samples apatite crystals were not detected even after 21 days of immersion in SBF. The apatite layer was characterized by scanning electron microscopy (SEM), EDS analysis and attenuated total reflectance-Fourier transform infrared spectrometry (FTIR-ATR). Weight loss measurements showed that the in vitro degradation rate of the composite scaffolds in SBF was higher in comparison to uncoated polyurethane samples. PUR and PUR/PDLLA foams with Bioglass coating have potential to be used as bioactive, biodegradable scaffolds in bone tissue engineering.
制备了用于骨组织工程的涂覆有生物玻璃颗粒的聚氨酯（PUR）和聚氨酯/聚（d，l-丙交酯）酸（PUR/PDLLA）基支架。采用浆料浸渍法制备涂层。PUR和PUR/PDLLA泡沫表面生物玻璃涂层的均匀结构表明，生物活性玻璃颗粒与聚氨酯具有良好的附着力，无需任何额外的表面处理。在模拟体液（SBF）中进行了体外研究，以研究生物玻璃涂层对PUR基支架生物降解性和生物活性的影响。在SBF中浸泡7天后，生物玻璃涂层样品的表面被一层含碳酸盐的磷灰石覆盖，而未涂层的聚合物样品即使在SBF中浸泡21天后也未检测到磷灰石晶体。通过扫描电子显微镜（SEM）、能谱分析（EDS）和衰减全反射-傅里叶变换红外光谱（FTIR-ATR）对磷灰石层进行了表征。失重测量表明，与未涂层的聚氨酯样品相比，复合支架在SBF中的体外降解速率更高。涂覆有生物玻璃的PUR和PUR/PDLLA泡沫有潜力用作骨组织工程中的生物活性、可生物降解支架。
