泰国H1N1、H1N2和H3N2猪流感病毒的基因特征分析

Genetic characterization of H1N1, H1N2 and H3N2 swine influenza virus in Thailand.

作者信息

Chutinimitkul Salin, Thippamom Nattakarn, Damrongwatanapokin Sudarat, Payungporn Sunchai, Thanawongnuwech Roongroje, Amonsin Alongkorn, Boonsuk Pitirat, Sreta Donruethai, Bunpong Napawan, Tantilertcharoen Rachod, Chamnanpood Pornchai, Parchariyanon Sujira, Theamboonlers Apiradee, Poovorawan Yong

机构信息

Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

出版信息

Arch Virol. 2008;153(6):1049-56. doi: 10.1007/s00705-008-0097-7. Epub 2008 May 6.

DOI:10.1007/s00705-008-0097-7

PMID:18458812

Abstract

Swine have been known to be a suitable host for influenza A virus. In Thailand, phylogenetic analysis on swine influenza virus (SIV) has as yet not been attempted. The present report presents molecular and phylogenetic analysis performed on SIV in Thailand. In this study, 12 SIV isolates from the central and eastern part of Thailand were subtyped and the molecular genetics of hemagglutinin and neuraminidase were elucidated. Three subtypes, H1N1, H1N2 and H3N2, are described. Phylogenetic analysis of the SIV hemagglutinin and neuraminidase genes shows individual clusters with swine, human or avian influenza virus at various global locations. Furthermore, amino acid substitutions were detected either at the receptor binding site or the antigenic sites of the hemagglutinin gene.

摘要

猪一直被认为是甲型流感病毒的合适宿主。在泰国，尚未尝试对猪流感病毒（SIV）进行系统发育分析。本报告介绍了对泰国猪流感病毒进行的分子和系统发育分析。在本研究中，对来自泰国中部和东部的12株猪流感病毒分离株进行了亚型鉴定，并阐明了血凝素和神经氨酸酶的分子遗传学。描述了三种亚型，即H1N1、H1N2和H3N2。猪流感病毒血凝素和神经氨酸酶基因的系统发育分析显示，在全球不同地点，与猪、人或禽流感病毒形成了单独的聚类。此外，在血凝素基因的受体结合位点或抗原位点检测到了氨基酸替换。