Charoenvisal Nataya, Keawcharoen Juthatip, Sreta Donruethai, Chaiyawong Supassama, Nonthabenjawan Nutthawan, Tantawet Siriporn, Jittimanee Suphattra, Arunorat Jirapat, Amonsin Alongkorn, Thanawongnuwech Roongroje
Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Road, Bangkok 10330, Thailand.
Virus Genes. 2013 Aug;47(1):75-85. doi: 10.1007/s11262-013-0927-x. Epub 2013 Jun 6.
Pandemic H1N1 2009 (pH1N1), influenza virus containing triple reassortant internal genes (TRIG) from avian, human, and swine influenza viruses emerged in 2009 as a highly infectious virus that was able to be transmitted from humans to pigs. During June 2010-May 2012, influenza virus surveillance was conducted in Thai pig population. Twenty-three samples (1.75%) were successfully isolated from total of 1,335 samples. Interestingly, pH1N1 (7 isolates, 30.34%), reassortant pH1N1 (rH1N1) (1 isolate, 4.35%), Thai endemic H1N1 (enH1N1) (3 isolates, 13.04%), reassortant H3N2 with pH1N1 internal genes (rH3N2) (9 isolates, 39.13%), and reassortant H1N2 with pH1N1 internal genes (rH1N2) (3 isolates, 13.04%) were found. It should be noted that rH1N1, rH1N2, and rH3N2 viruses contained the internal genes of pH1N1 virus having a TRIG cassette descendant from the North American swine lineage. Although all isolates in this study were obtained from mild clinically sick pigs, the viruses were still highly infective and possibly may play an important role in human-animal interfacing transmission. In addition, the TRIG cassette may have an influence on antigenic shift resulting in emergence of novel viruses, as seen in this study. Continuing surveillance of influenza A natural hosts, particularly in pigs is necessary.
2009年甲型H1N1流感大流行病毒（pH1N1）含有来自禽、人、猪流感病毒的三重重配内部基因（TRIG），于2009年出现，是一种具有高度传染性、能够在人猪之间传播的病毒。2010年6月至2012年5月期间，对泰国猪群进行了流感病毒监测。从1335份样本中成功分离出23份样本（1.75%）。有趣的是，发现了pH1N1（7株，30.34%）、重配pH1N1（rH1N1）（1株，4.35%）、泰国地方性H1N1（enH1N1）（3株，13.04%）、带有pH1N1内部基因的重配H3N2（rH3N2）（9株，39.13%）以及带有pH1N1内部基因的重配H1N2（rH1N2）（3株，13.04%）。应当指出的是，rH1N1、rH1N2和rH3N2病毒含有pH1N1病毒的内部基因，其具有源自北美猪系的TRIG盒。尽管本研究中的所有分离株均来自临床症状较轻的病猪，但这些病毒仍具有高度传染性，可能在人畜界面传播中发挥重要作用。此外，如本研究所示，TRIG盒可能对抗原转变有影响，导致新病毒出现。持续监测甲型流感的天然宿主，尤其是猪，是必要的。
