De Rosa C M, Ozzello L, Greene G L, Habif D V
Arthur Purdy Stout Laboratory of Surgical Pathology, Columbia University, New York, New York.
Am J Surg Pathol. 1987 Dec;11(12):943-50. doi: 10.1097/00000478-198712000-00004.
Immunostaining of estrogen receptor was carried out on paraffin sections of breast carcinomas using an anti-estrophilin monoclonal antibody (D75P3 gamma) and the avidin-biotin technique. The tumors were fixed in Bouin's solution or in formalin for varying periods of time at room temperature or at 4 degrees C. Best results were obtained following fixation in Bouin's at room temperature or in formalin at 4 degrees C. The staining was localized in the nuclei of carcinoma cells and was heterogeneous in intensity and extent. Prolonged fixation resulted in decreased immunoreactivity and in the appearance of nonspecific cytoplasmic and background staining. The estrogen receptor immunostaining on paraffin sections was found to be in concordance with that on frozen sections (Abbott ER-ICA) and with the steroid-binding assay (dextran-coated charcoal) in over 90% of the cases. This method is of easy and rapid execution and yields reliable and reproducible results.
使用抗雌激素受体单克隆抗体（D75P3γ）和抗生物素蛋白-生物素技术，对乳腺癌石蜡切片进行雌激素受体免疫染色。肿瘤在室温或4℃下，用布安氏液或福尔马林固定不同时间。在室温下用布安氏液固定或在4℃下用福尔马林固定后，可获得最佳结果。染色定位于癌细胞核，强度和范围不均一。长时间固定会导致免疫反应性降低，并出现非特异性细胞质和背景染色。发现石蜡切片上的雌激素受体免疫染色与冰冻切片（雅培ER-ICA）和类固醇结合试验（葡聚糖包被活性炭）在90%以上的病例中一致。该方法操作简便、快速，结果可靠且可重复。
