结直肠癌患者血浆中VEGF-A mRNA水平作为血管生成可能替代标志物的研究
Levels of VEGF-A mRNA in plasma from patients with colorectal carcinoma as possible surrogate marker of angiogenesis.
作者信息
Garcia Vanesa, Garcia Jose M, Silva Javier, Peña Cristina, Dominguez Gemma, Lorenzo Yolanda, Diaz Raquel, Alonso Isabel, Colas Antonio, Hurtado Alicia, Sanchez Antonio, Bonilla Felix
机构信息
Department of Medical Oncology, Hospital Universitario Puerta de Hierro, C/San Martín de Porres, 4, 28035, Madrid, Spain.
出版信息
J Cancer Res Clin Oncol. 2008 Nov;134(11):1165-71. doi: 10.1007/s00432-008-0405-3. Epub 2008 May 7.
PURPOSE
The regulator of angiogenesis most extensively studied is VEGF. VEGF mRNA in plasma from patients with colorectal cancer was analyzed as a possible surrogate marker of tumor angiogenesis.
METHODS
VEGF mRNA was measured by quantitative PCR in plasma, tumors and circulating tumor cells from colorectal cancer patients. Circulating VEGF protein was analyzed by ELISA. Microvessel density was determined.
RESULTS
Levels of VEGF mRNA and protein in plasma were higher in patients than in controls. VEGF mRNA was overexpressed in tumors with respect to normal tissues. Levels of VEGF protein were associated with VEGF mRNA in plasma, but no associations with tumor samples were found. A trend to statistical significance was shown between high VEGF mRNA and vascular invasion. MVD was not related to VEGF mRNA in plasma.
CONCLUSIONS
Thus, VEGF mRNA could be a marker similar to VEGF protein in plasma.
目的
研究最为广泛的血管生成调节因子是血管内皮生长因子（VEGF）。分析结直肠癌患者血浆中的VEGF mRNA，将其作为肿瘤血管生成的一种可能替代标志物。
方法
采用定量PCR法检测结直肠癌患者血浆、肿瘤组织及循环肿瘤细胞中的VEGF mRNA。通过酶联免疫吸附测定（ELISA）分析循环VEGF蛋白。测定微血管密度。
结果
患者血浆中VEGF mRNA和蛋白水平高于对照组。与正常组织相比，肿瘤组织中VEGF mRNA过度表达。血浆中VEGF蛋白水平与VEGF mRNA相关，但未发现与肿瘤样本有关联。高VEGF mRNA与血管侵犯之间呈现出统计学意义的趋势。微血管密度与血浆中的VEGF mRNA无关。
结论
因此，VEGF mRNA可能是血浆中与VEGF蛋白类似的一种标志物。
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