Granger Jennifer H, Williams Rebecca, Lenz Eva M, Plumb Robert S, Stumpf Chris L, Wilson Ian D
AstraZeneca R&D Boston, 35 Gatehouse Drive, Waltham, MA 02451, USA.
Rapid Commun Mass Spectrom. 2007;21(13):2039-45. doi: 10.1002/rcm.3059.
Ultra-performance liquid chromatography (UPLC) coupled to orthogonal acceleration time-of-flight mass spectrometry (oa-TOFMS) in positive electrospray ionization mode was used to obtain metabolite profiles for urine obtained from three strains of Zucker rat. These were the Zucker lean, the Zucker (fa/fa) obese and the Zucker lean/(fa) cross. Clear age- and strain-related differences were noted with the leptin-deficient (fa/fa) obese animal showing significant differences from both the other Zucker rat strains with respect to metabolite profiles.
采用超高效液相色谱（UPLC）与正电喷雾电离模式下的正交加速飞行时间质谱（oa-TOFMS）联用，来获取来自三种品系Zucker大鼠尿液的代谢物谱。这三种品系分别是Zucker瘦鼠、Zucker（fa/fa）肥胖鼠以及Zucker瘦鼠/（fa）杂交鼠。研究发现，代谢物谱存在明显的年龄和品系相关差异，其中瘦素缺乏的（fa/fa）肥胖动物与其他两种Zucker大鼠品系在代谢物谱方面存在显著差异。
