Plumb Robert S, Granger Jennifer H, Stumpf Chris L, Johnson Kelly A, Smith Brian W, Gaulitz Scott, Wilson Ian D, Castro-Perez Jose
Waters Corporation, 34 Maple Street, Milford, MA 01757, USA.
Analyst. 2005 Jun;130(6):844-9. doi: 10.1039/b501767j. Epub 2005 Apr 27.
The use of Ultra Performance Liquid Chromatography (UPLC), with a rapid 1.5 minute reversed-phase gradient separation on a 1.7 microm reversed-phase packing material to provide rapid "high throughput" support for metabonomic screening is demonstrated. The peak capacity and the number of marker ions detected using these fast UPLC separations and oa-TOF MS was found to be similar to that generated by conventional HPLC-MS methods with a 10 minute separation. The UPLC-MS methodology was applied to the analysis of urine samples from rodents, including normal and Zucker obese rats and three strains of mice (of both sexes), and was found to provide rapid discrimination between age, strain, gender and diurnal variation.
本文展示了超高效液相色谱（UPLC）的应用，其在1.7微米反相填充材料上进行1.5分钟的快速反相梯度分离，可为代谢组学筛选提供快速的“高通量”支持。使用这些快速UPLC分离和oa-TOF MS检测到的峰容量和标记离子数量，与采用10分钟分离的传统HPLC-MS方法所产生的结果相似。UPLC-MS方法被应用于分析来自啮齿动物（包括正常和Zucker肥胖大鼠以及三种品系的小鼠（雌雄均有））的尿液样本，结果发现该方法能够快速区分年龄、品系、性别和昼夜变化。
