Wang Yuan, Lu Xin, Xu Guowang
National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
J Sep Sci. 2008 May;31(9):1564-72. doi: 10.1002/jssc.200700663.
In the present study, we developed a novel online hydrophilic interaction chromatography (HILIC)-RPLC separation system for simultaneous separation of both hydrophilic and hydrophobic solutes in a complex sample with one injection. A HILIC Si column and a C18 column were hyphenated with an interface including two electronic 2-position valves, a solvent pump, and a solute transfer column. By using column-switching technique, the nonretained hydrophobic solutes were eluted out of the HILIC column and transferred into the C18 column to perform further separation. The hydrophilic solutes were separated on the HILIC column at the same time. One detector was equipped for each column to record the individual chromatograms. By separating a standard mixture and a traditional Chinese medicine (TCM) extract, the developed HILIC-RPLC system demonstrates its potential for "entire-components" separation of complex samples with improved peak capacity and throughput compared with the common single-column LC.
在本研究中，我们开发了一种新型的在线亲水相互作用色谱（HILIC）-反相液相色谱（RPLC）分离系统，用于通过一次进样同时分离复杂样品中的亲水性和疏水性溶质。一根HILIC硅胶柱和一根C18柱通过一个包含两个二位电子阀、一个溶剂泵和一个溶质转移柱的接口进行联用。通过使用柱切换技术，未保留的疏水性溶质从HILIC柱中洗脱出来并转移到C18柱中进行进一步分离。同时，亲水性溶质在HILIC柱上进行分离。每根柱配备一个检测器以记录各自的色谱图。通过分离标准混合物和中药提取物，所开发的HILIC-RPLC系统展示了其在复杂样品“全成分”分离方面的潜力，与普通单柱液相色谱相比，具有更高的峰容量和通量。
