Wang Yuan, Lehmann Rainer, Lu Xin, Zhao Xinjie, Xu Guowang
National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, China.
J Chromatogr A. 2008 Sep 12;1204(1):28-34. doi: 10.1016/j.chroma.2008.07.010. Epub 2008 Jul 11.
In this study we developed and optimized a column-switching high-performance liquid chromatographic (HPLC) system for the complementary analysis of polar and apolar compounds in complex samples. The polar compounds are separated on a hydrophilic interaction chromatographic (HILIC) column; the part of the sample non-retained on HILIC is transferred into and separated on a reversed-phase (RP) column through an interface, thus preventing the loss of analytes eluting at dead time in common single-column mode. The signals are in turn recorded in the same chromatogram either by a UV or by a mass spectrometric detector. Applying a mixture of 25 standard compounds (12 hydrophilic and 13 hydrophobic) the system proves to be reliable, robust and easy-operating. The investigation of urine revealed an increase in the detected metabolite ion masses compared to the single-column HILIC-electrospray ionization (ESI)-MS analysis. Utilizing online the complementary selectivity of HILIC and RPLC in a fully automatic mode, this approach holds out the prospect to enlarge the number of detectable compounds in non-targeted "-omics" studies and sophisticated target-driven approaches by a single injection.
在本研究中，我们开发并优化了一种柱切换高效液相色谱（HPLC）系统，用于复杂样品中极性和非极性化合物的互补分析。极性化合物在亲水作用色谱（HILIC）柱上分离；HILIC上未保留的样品部分通过接口转移至反相（RP）柱上并在该柱上分离，从而避免了在常规单柱模式下死时间洗脱的分析物损失。信号依次由紫外或质谱检测器记录在同一张色谱图中。应用包含25种标准化合物（12种亲水和13种疏水）的混合物，该系统被证明可靠、稳健且易于操作。尿液研究表明，与单柱HILIC-电喷雾电离（ESI）-MS分析相比，检测到的代谢物离子质量有所增加。以全自动模式在线利用HILIC和RPLC的互补选择性，该方法有望通过一次进样增加非靶向“组学”研究和复杂目标驱动方法中可检测化合物的数量。
