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人免疫缺陷病毒1型长末端重复序列/猴病毒40早期区域融合基因在转基因小鼠中的表达

Expression of a human immunodeficiency virus type 1 long terminal repeat/simian virus 40 early region fusion gene in transgenic mice.

作者信息

Skowronski J

机构信息

Cold Spring Harbor Laboratory, New York 11724.

出版信息

J Virol. 1991 Feb;65(2):754-62. doi: 10.1128/JVI.65.2.754-762.1991.

DOI:10.1128/JVI.65.2.754-762.1991
PMID:1846196
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC239815/
Abstract

Three lines of transgenic mice carrying the human immunodeficiency virus type 1 (HIV-1) long terminal repeat fused to the simian virus 40 early region (HIV-1 Tag) were constructed. Expression of the transgenes was reproducibly observed in the lymphoid tissue and skin of all three transgenic lines studied. Interestingly, cell types other than T cells, i.e., B cells and thymic stromal cells, contributed most of the expression detectable in the lymphoid organs. Each transgenic line also displayed a different but consistent pattern of transgene expression in nonlymphoid organs. These individual patterns probably reflect the effects of particular chromosomal integration sites on transcriptional activity of the HIV-1 promoter.

摘要

构建了三系携带与猿猴病毒40早期区域融合的人类免疫缺陷病毒1型（HIV-1）长末端重复序列（HIV-1 Tag）的转基因小鼠。在所研究的所有三个转基因品系的淋巴组织和皮肤中均可重复观察到转基因的表达。有趣的是，除T细胞外的其他细胞类型，即B细胞和胸腺基质细胞，在淋巴器官中贡献了大部分可检测到的表达。每个转基因品系在非淋巴器官中也表现出不同但一致的转基因表达模式。这些个体模式可能反映了特定染色体整合位点对HIV-1启动子转录活性的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/549e/239815/6bb2cf995482/jvirol00045-0214-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/549e/239815/71aac82965ab/jvirol00045-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/549e/239815/ac6a32062db7/jvirol00045-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/549e/239815/ccbaf704a4f3/jvirol00045-0213-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/549e/239815/6bb2cf995482/jvirol00045-0214-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/549e/239815/71aac82965ab/jvirol00045-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/549e/239815/ac6a32062db7/jvirol00045-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/549e/239815/ccbaf704a4f3/jvirol00045-0213-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/549e/239815/6bb2cf995482/jvirol00045-0214-a.jpg

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Expression of a human immunodeficiency virus type 1 long terminal repeat/simian virus 40 early region fusion gene in transgenic mice.人免疫缺陷病毒1型长末端重复序列/猴病毒40早期区域融合基因在转基因小鼠中的表达
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