Jolicoeur P, Laperrière A, Beaulieu N
Laboratory of Molecular Biology, Clinical Research Institute of Montreal, Québec, Canada.
J Virol. 1992 Jun;66(6):3904-8. doi: 10.1128/JVI.66.6.3904-3908.1992.
Transgenic mice containing the complete human immunodeficiency virus (HIV) coding sequences fused to the mouse mammary tumor virus long terminal repeat were generated. They were found to produce high levels of authentic gag and env HIV proteins in several tissues known to support mouse mammary tumor virus-driven transcription. HIV proteins were also detected in serum and in body fluids (milk and epididymal secretions) known to be natural sites of retrovirus, and specifically of HIV, production. These results indicate that primary mouse cells from different tissues have the capacity to produce HIV proteins. These mice represent a novel animal model for HIV infection.
构建了转基因小鼠，其含有与小鼠乳腺肿瘤病毒长末端重复序列融合的完整人类免疫缺陷病毒（HIV）编码序列。发现在已知支持小鼠乳腺肿瘤病毒驱动转录的多个组织中，它们能产生高水平的真实HIV gag和env蛋白。在血清以及已知是逆转录病毒，特别是HIV产生天然部位的体液（乳汁和附睾分泌物）中也检测到了HIV蛋白。这些结果表明，来自不同组织的原代小鼠细胞有产生HIV蛋白的能力。这些小鼠代表了一种新型的HIV感染动物模型。
