Steffy K R, Weir J P
Department of Microbiology, University of Tennessee, Knoxville 37996.
J Virol. 1991 Feb;65(2):972-5. doi: 10.1128/JVI.65.2.972-975.1991.
To investigate the cis-acting sequence elements that are involved in the regulation of herpes simplex virus type 1 late-gene expression, recombinant viruses were constructed that express the Escherichia coli lacZ gene from the promoter of the glycoprotein H (gH) gene. Deletion experiments established an upstream boundary for the gH promoter of no more than 83 bp from the start of gH transcription and showed that the promoter sequences did not overlap with coding sequences of the upstream thymidine kinase (tk) gene. Sequences of the tk gene previously shown to be required for efficient processing of the tk transcript were essential for expression form the gH promoter and included a TATA-like element. In addition, the gH TATA element was specifically mutagenized to substitute the TATA elements of immediate-early, early, and other late viral promoters for the gH TATA element. The results indicated that the TATA element was an interchangeable component of herpes simplex virus type 1 promoters and did not regulate temporal expression.
为了研究参与单纯疱疹病毒1型晚期基因表达调控的顺式作用序列元件，构建了从糖蛋白H（gH）基因启动子表达大肠杆菌lacZ基因的重组病毒。缺失实验确定了gH启动子的上游边界距离gH转录起始点不超过83 bp，并表明启动子序列与上游胸苷激酶（tk）基因的编码序列不重叠。先前显示对tk转录本有效加工所必需的tk基因序列对于gH启动子的表达至关重要，并且包括一个类TATA元件。此外，gH TATA元件被特异性诱变，以将即刻早期、早期和其他晚期病毒启动子的TATA元件替换为gH TATA元件。结果表明，TATA元件是单纯疱疹病毒1型启动子的一个可互换成分，并不调节时间表达。
