白细胞介素-1β影响星形胶质细胞祖细胞的钙信号传导和体外细胞迁移。
Interleukin-1beta affects calcium signaling and in vitro cell migration of astrocyte progenitors.
作者信息
Striedinger Katharine, Scemes Eliana
机构信息
The Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
出版信息
J Neuroimmunol. 2008 May 30;196(1-2):116-23. doi: 10.1016/j.jneuroim.2008.03.014. Epub 2008 May 6.
Spontaneous calcium activity of neural progenitors is largely dependent on a paracrine signaling mechanism involving release of ATP and activation of purinergic receptors. Although it is well documented that, in mature astrocytes, cytokines modulate the expression levels of certain purinergic receptors, nothing is known about their impact during early stages of development. Here we provide evidence that conditioned medium from activated microglia and interleukin-1beta, but not tumor necrosis factor-alpha, decrease the frequency of calcium oscillations and reduce the rate of in vitro migration of astrocyte progenitors. Such alterations were due to changes in activity of two purinergic P2 receptors, and not to the amount of released ATP. These results indicate that interleukin-1beta plays an important role during early stages of CNS development, modulating calcium signaling and cell migration.
神经祖细胞的自发钙活性在很大程度上依赖于一种旁分泌信号机制，该机制涉及三磷酸腺苷（ATP）的释放和嘌呤能受体的激活。尽管有充分的文献记载，在成熟星形胶质细胞中，细胞因子可调节某些嘌呤能受体的表达水平，但关于它们在发育早期阶段的影响却一无所知。在这里，我们提供证据表明，活化小胶质细胞的条件培养基和白细胞介素-1β，但不是肿瘤坏死因子-α，会降低钙振荡的频率，并降低星形胶质细胞祖细胞的体外迁移率。这种改变是由于两种嘌呤能P2受体活性的变化，而不是由于ATP的释放量。这些结果表明，白细胞介素-1β在中枢神经系统发育的早期阶段发挥重要作用，调节钙信号和细胞迁移。
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