一种减毒活严重急性呼吸综合征冠状病毒在叙利亚金黄地鼠中具有免疫原性且有效。
A live attenuated severe acute respiratory syndrome coronavirus is immunogenic and efficacious in golden Syrian hamsters.
作者信息
Lamirande Elaine W, DeDiego Marta L, Roberts Anjeanette, Jackson Jadon P, Alvarez Enrique, Sheahan Tim, Shieh Wun-Ju, Zaki Sherif R, Baric Ralph, Enjuanes Luis, Subbarao Kanta
机构信息
Laboratory of Infectious Diseases, NIAID, NIH, Bethesda, MD 20892, USA.
出版信息
J Virol. 2008 Aug;82(15):7721-4. doi: 10.1128/JVI.00304-08. Epub 2008 May 7.
Abstract
The immunogenicity and protective efficacy of a live attenuated vaccine consisting of a recombinant severe acute respiratory syndrome (SARS) coronavirus lacking the E gene (rSARS-CoV-DeltaE) were studied using hamsters. Hamsters immunized with rSARS-CoV-DeltaE developed high serum-neutralizing antibody titers and were protected from replication of homologous (SARS-CoV Urbani) and heterologous (GD03) SARS-CoV in the upper and lower respiratory tract. rSARS-CoV-DeltaE-immunized hamsters remained active following wild-type virus challenge, while mock-immunized hamsters displayed decreased activity. Despite being attenuated in replication in the respiratory tract, rSARS-CoV-DeltaE is an immunogenic and efficacious vaccine in hamsters.
摘要
利用仓鼠研究了一种缺失E基因的重组严重急性呼吸综合征（SARS）冠状病毒减毒活疫苗（rSARS-CoV-DeltaE）的免疫原性和保护效力。用rSARS-CoV-DeltaE免疫的仓鼠产生了高血清中和抗体滴度，并在上、下呼吸道免受同源（SARS-CoV Urbani）和异源（GD03）SARS-CoV的复制。rSARS-CoV-DeltaE免疫的仓鼠在受到野生型病毒攻击后仍保持活跃，而模拟免疫的仓鼠活动减少。尽管rSARS-CoV-DeltaE在呼吸道中的复制能力减弱，但它在仓鼠中是一种具有免疫原性且有效的疫苗。
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