Genome. 1995 Feb;38(1):36-44. doi: 10.1139/g95-005.
A F2 population of two celery cultivated types (Apium graveolens L. var. rapaceum and A. graveolens L. var. secalinum) was used to construct a linkage map consisting of 29 RFLP (restriction fragment length polymorphism), 100 RAPD (random amplified polymorphic DNA), four isozyme, one disease resistance, and one growth habit markers. The map contains 11 major groups and 9 small groups and has a total length of 803 cM with an average distance of 6.4 cM between two adjacent loci. Ten percent of the RAPDs segregated as codominant markers and their allelic homologies were tested by Southern hybridization. One-quarter of the dominant RAPDs were linked in repulsion phase, whereas the majority of them were linked to either codominant or dominant markers in coupling phase. About 10% of the markers showed significant segregation distortion. The detectable level of duplications in the celery genome was relatively low.
利用 2 个普通芹菜品种(Apium graveolens L. var. rapaceum 和 A. graveolens L. var. secalinum)的 F2 群体,构建了一张包含 29 个 RFLP(限制性片段长度多态性)、100 个 RAPD(随机扩增多态性 DNA)、4 个同工酶、1 个抗病性和 1 个生长习性标记的连锁图谱。图谱包含 11 个大组和 9 个小组,总长度为 803cM,两个相邻标记之间的平均距离为 6.4cM。10%的 RAPD 分离为共显性标记,并通过 Southern 杂交检测其等位基因同源性。四分之一的显性 RAPD 呈相斥相连锁,而大多数呈相斥或相吸连锁。约 10%的标记出现明显的分离失真。芹菜基因组中的重复检测水平相对较低。