Genome. 1995 Apr;38(2):255-64. doi: 10.1139/g95-032.
A genetic linkage map of Brassica nigra, comprised of 288 loci in eight linkage groups, was constructed. The linkage groups varied in size from 72 to 159 cM and the total map length was 855 cM. The recurrent parent used in the backcross was extremely heterozygous. This allowed recombination to be estimated separately for female (recurrent parent) meiosis and male (F1) meiosis over a large proportion of the genome. Significant differences between male and female recombination frequencies were observed on all six linkage groups where data was available for both sexes. Enhanced male recombination frequencies were observed that were associated with proterminal regions, while enhanced female recombination frequencies were adjacent to putative centromeres. It is possible that the distinct genotypes of the F1 (male) and recurrent (female) parents contributed to the observed differences in recombination. However, this study emphasizes the need to consider potential sex differences, in both the rate and the position of recombination, when planning genetic experiments and breeding programmes.
构建了包含 8 个连锁群 288 个位点的芸薹属黑芥遗传连锁图谱。连锁群的大小从 72 到 159cM 不等，图谱总长度为 855cM。回交所用的轮回亲本高度杂合。这使得重组能够在基因组的很大一部分上分别估计雌性（轮回亲本）减数分裂和雄性（F1）减数分裂的情况。在两性均有数据的 6 个连锁群上，均观察到雄性和雌性重组频率之间存在显著差异。在近端区域观察到增强的雄性重组频率，而在假定着丝粒附近观察到增强的雌性重组频率。F1（雄性）和轮回（雌性）亲本的不同基因型可能导致了观察到的重组差异。然而，这项研究强调了在规划遗传实验和育种计划时，需要考虑重组速率和位置上的潜在性别差异。
