Genome. 1995 Apr;38(2):298-306. doi: 10.1139/g95-037.
This study was undertaken to assess the relative level of molecular diversity between cultivated barley, Hordeum vulgare ssp. vulgare (HV), and one of its wild relatives, H. vulgare ssp. spontaneum (HS), and to identify possible restriction fragment length polymorphism (RFLP) patterns that may provide information concerning the phylogenetic relationship between these two barley groups. A total of 363 barley accessions were assayed, including 95 entries of HV collected from 36 major barley growing countries of the world and 268 entries of HS from 25 natural populations in Israel and Iran. The 26 RFLP marker loci used in the survey represent single-copy, low-copy, and repetitive DNA sequences and mark all of the chromosome arms. A randomization test, on the basis of equal sample sizes, showed that HS is more polymorphic than HV, as evaluated by the number of alleles and diversity indices. The analysis also indicated extensive RFLP differentiation between these two barley groups; highly significant differences of allele frequencies were detected at the majority of the loci. The HV sample can be subdivided according to winter or spring growth habits, and two- or six-rowed spikes. Analysis of genetic polymorphisms in these subgroups showed that levels of diversity were about equal in spring and winter groups and also in the groups with two- and six-rowed spikes. However, significant differences of allelic frequencies were detected between subgroups of the two divisions.
本研究旨在评估栽培大麦（Hordeum vulgare ssp. vulgare，HV）与其野生近缘种（H. vulgare ssp. spontaneum，HS）之间相对分子多样性水平，并鉴定可能提供有关这两个大麦群体间系统发育关系信息的限制性片段长度多态性（RFLP）模式。共对 363 个大麦品系进行了分析，其中包括从世界 36 个主要大麦种植国家收集的 95 个 HV 品系和从以色列和伊朗的 25 个自然种群收集的 268 个 HS 品系。调查中使用的 26 个 RFLP 标记位点代表单拷贝、低拷贝和重复 DNA 序列，标记所有染色体臂。基于等样本量的随机化检验表明，HS 的多态性高于 HV，这可通过等位基因数和多样性指数来评估。分析还表明，这两个大麦群体之间存在广泛的 RFLP 分化；在大多数位点都检测到等位基因频率的显著差异。可根据冬、春生长习性和二棱或六棱穗对 HV 样本进行细分。对这些亚组遗传多态性的分析表明，春冬组以及二棱和六棱穗组的多样性水平大致相当。然而，在这两个分组的亚组之间检测到等位基因频率的显著差异。
