Neale D B, Saghai-Maroof M A, Allard R W, Zhang Q, Jorgensen R A
Department of Genetics, University of California, Davis 95616.
Genetics. 1988 Dec;120(4):1105-10. doi: 10.1093/genetics/120.4.1105.
Chloroplast DNA (cpDNA) diversity was found within and among populations (245 accessions total) of wild barley, Hordeum vulgare L. ssp. spontaneum Koch from Israel and Iran. Three polymorphic restriction sites (HindIII, EcoRI, BclI) which define three distinct cpDNA lineages were detected. One lineage is common to populations in the Hule Valley and Kinneret of northern Israel, and in Iran. The second lineage is found predominantly in the Lower Jordan Valley and Negev. The distribution of the third lineage is scattered but widespread throughout Israel. Sixty two accessions of cultivated barleys, H. vulgare L., were found, with two exceptions, to belong to just one cpDNA lineage of wild barley, indicating that the cpDNA of cultivated barley is less variable than its wild ancestor. These results demonstrate the need for assessing intraspecific cpDNA variability prior to choosing single accessions for phylogenetic constructions at the species level and higher.
在来自以色列和伊朗的野生大麦（Hordeum vulgare L. ssp. spontaneum Koch）群体内部和群体之间发现了叶绿体DNA（cpDNA）多样性（总共245份材料）。检测到三个多态性限制位点（HindIII、EcoRI、BclI），它们定义了三个不同的cpDNA谱系。一个谱系在以色列北部的胡勒谷和基尼烈以及伊朗的群体中是共有的。第二个谱系主要出现在约旦河谷下游和内盖夫地区。第三个谱系的分布较为分散，但在以色列各地广泛存在。发现62份栽培大麦（H. vulgare L.）材料，除了两份例外，都只属于野生大麦的一个cpDNA谱系，这表明栽培大麦的cpDNA变异性低于其野生祖先。这些结果表明，在为物种水平及更高水平的系统发育构建选择单个材料之前，需要评估种内cpDNA变异性。
