Shi Guo-Ming, Xu Yang, Fan Jia, Zhou Jian, Yang Xin-Rong, Qiu Shuang-Jian, Liao Yong, Wu Wei-Zhong, Ji Yuan, Ke Ai-Wu, Ding Zhen-Bin, He Yi-Zhou, Wu Bing, Yang Guo-Huan, Qin Wen-Zhen, Zhang Wu, Zhu Jiang, Min Zhi-Hui, Wu Zhi-Quan
Key Laboratory for Carcinogenesis and Cancer Invasion, Liver Cancer Institute, Zhongshan Hospital and Shanghai Medical School, Fudan University, the Chinese Ministry of Education, 136 Yi Xue Yuan Road, Shanghai, 200032, People's Republic of China.
J Cancer Res Clin Oncol. 2008 Nov;134(11):1155-63. doi: 10.1007/s00432-008-0407-1. Epub 2008 May 10.
To identify the side population (SP) cells from four hepatocellular carcinoma (HCC) cell lines with stepwise metastatic potentials.
SP cells were sorted from HCCLM3, MHCC97-H, MHCC97-L and Hep3B by flow cytometry, and then analyzed by differentiation study, clonogenic assay, chemoresistance study and tumorigenicity assay in vivo. The expression of ABCG(2) in SP cells was detected by immunocytochemistry, western blotting and real-time quantitative PCR, respectively.
There was significant difference in SP proportion among HCCLM3, MHCC97-H, MHCC97-L and Hep3B (28.7 +/- 1.6%, 14.5 +/- 0.6%, 4.2 +/- 0.4%, 0.9 +/- 0.1%, respectively, P < 0.01). All the SP cells showed similar characteristics of self-renewal, high clonogenicity, remarkable chemo-resistance and high expression of ABCG(2). As low as 2,000 SP cells could initiate tumors in non-obese diabetic/severe combined immunodeficiency mice successfully.
SP cells purified from HCC cell lines harbors cancer stem cell-like properties, and may be related to the metastatic potentials and therapeutic-resistance of HCC.
从具有逐步转移潜能的四种肝癌（HCC）细胞系中鉴定侧群（SP）细胞。
通过流式细胞术从HCCLM3、MHCC97-H、MHCC97-L和Hep3B细胞系中分选SP细胞，然后通过分化研究、克隆形成试验、化疗耐药性研究和体内致瘤性试验进行分析。分别采用免疫细胞化学、蛋白质印迹法和实时定量PCR检测SP细胞中ABCG(2)的表达。
HCCLM3、MHCC97-H、MHCC97-L和Hep3B细胞系的SP比例存在显著差异（分别为28.7±1.6%、14.5±0.6%、4.2±0.4%、0.9±0.1%，P<0.01）。所有SP细胞均表现出相似的自我更新、高克隆形成能力、显著的化疗耐药性和ABCG(2)高表达特征。低至2000个SP细胞就能在非肥胖糖尿病/严重联合免疫缺陷小鼠中成功引发肿瘤。
从肝癌细胞系中纯化的SP细胞具有癌干细胞样特性，可能与肝癌的转移潜能和治疗耐药性有关。
