Department of Immunology Institute of Biomedical Sciences University of São Paulo São Paulo Brazil.
Mediators Inflamm. 1997;6(2):127-33. doi: 10.1080/09629359791820.
We describe here a novel experimental model of late-phase reaction of immediate hypersensitivity developed in mice. It consists of introducing small fragments of heat-coagulated hen egg white into the subcutaneous tissue of mice. After 14 days, animals challenged with purified ovalbumin into the footpad presented an immediate swelling of the paw peaking at 30 min, followed by two peaks of swelling at 6 and 24 h. Histological examination of the paws showed a massive eosinophil infiltration (more than 800 cells/5 microscopic fields). This intense infiltration persisted for more than 14 days after the challenge. Furthermore, in mice immunized with coagulated egg white the delayed swelling of the paws and eosinophilic infiltration were significantly higher than in mice immunized with the classical protocol of ovalbumin in alumen adjuvant. Transfer of lymph node cells obtained from mice implanted with heat-coagulated hen egg white induced footpad swelling and eosinophil infiltration in response to ovalbumin. High levels of ovalbuminspecific IgG1 but not of IgE were detected in the serum of these animals. The advantages of this model for the experimental study of late-phase reaction per se and its relevance to the study of allergic diseases such as asthma are discussed.
我们在这里描述了一种在小鼠中诱导迟发型过敏反应的新型实验模型。该模型由将热凝结的鸡卵白蛋白小片段引入皮下组织组成。14 天后，用纯化卵清蛋白对动物进行足底挑战，会导致足部立即肿胀，在 30 分钟达到峰值，随后在 6 和 24 小时出现两个肿胀高峰。对足部的组织学检查显示大量嗜酸性粒细胞浸润（超过 5 个显微镜视野中的 800 个细胞）。这种强烈的浸润在挑战后持续超过 14 天。此外，在用热凝结卵清蛋白免疫的小鼠中，与用卵清蛋白在明矾佐剂中的经典方案免疫的小鼠相比，迟发性足部肿胀和嗜酸性粒细胞浸润明显更高。从植入热凝结鸡卵白蛋白的小鼠中获得的淋巴结细胞转移会引起针对卵清蛋白的足垫肿胀和嗜酸性粒细胞浸润。这些动物的血清中检测到高水平的卵清蛋白特异性 IgG1，但没有 IgE。讨论了该模型对迟发型反应本身的实验研究的优势及其与哮喘等过敏疾病研究的相关性。
