Allergy Unit University Hospital 1211 Geneva 14 Switzerland.
Mediators Inflamm. 1994;3(2):143-8. doi: 10.1155/S0962935194000189.
The effects of the bacterial extract OM-85 on the respiratory burst, intracellular calcium and the stress response have been investigated in human peripheral blood monocytes from normal donors. Activation of the respiratory burst during bacterial phagocytosis has been previously associated with heat shock/stress proteins synthesis. Whereas OM-85 stimulated superoxide production and increased Ca(2+) mobilization, it fared to induce synthesis of classical HSPs. The lack of stress protein induction was observed even in the presence of iron which potentiates both oxidative injury and stress protein induction during bacterial phagocytosis. However OM-85 induced a 75-78 kDa protein, which is likely to be a glucose regulated protein (GRP78), and enhanced intracellular expression of interleukin-lbeta precursor.
研究了细菌提取物 OM-85 对正常人外周血单核细胞呼吸爆发、细胞内钙和应激反应的影响。以前的研究表明，细菌吞噬过程中的呼吸爆发与热休克/应激蛋白的合成有关。虽然 OM-85 刺激超氧化物的产生并增加钙动员，但它不能诱导经典 HSPs 的合成。即使在铁存在的情况下，也观察到应激蛋白的诱导缺失，而铁能增强细菌吞噬过程中的氧化损伤和应激蛋白的诱导。然而，OM-85 诱导了一种 75-78 kDa 的蛋白质，很可能是一种葡萄糖调节蛋白（GRP78），并增强了白细胞介素-1β前体的细胞内表达。
