Uhlén Mathias
School of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm, Sweden.
Biotechniques. 2008 Apr;44(5):649-54. doi: 10.2144/000112803.
The use of affinity-based tools has become invaluable as a platform for basic research and in the development of drugs and diagnostics. Applications include affinity chromatography and affinity tag fusions for efficient purification of proteins as well as methods to probe the protein network interactions on a whole-proteome level. A variety of selection systems has been described for in vitro evolution of affinity reagents using combinatorial libraries, which make it possible to create high-affinity reagents to virtually all biomolecules, as exemplified by generation of therapeutic antibodies and new protein scaffold binders. The strategies for high-throughput generation of affinity reagents have also opened up the possibility of generating specific protein probes on a whole-proteome level. Recently, such affinity proteomics have allowed the detailed analysis of human protein expression in a comprehensive manner both in normal and disease tissue using tissue microarrays and confocal microscopy.
基于亲和力的工具作为基础研究以及药物和诊断开发的平台，其用途已变得至关重要。应用包括用于高效纯化蛋白质的亲和色谱法和亲和标签融合技术，以及在全蛋白质组水平上探测蛋白质网络相互作用的方法。已经描述了多种利用组合文库进行亲和试剂体外进化的筛选系统，这使得几乎可以针对所有生物分子创建高亲和力试剂，治疗性抗体和新型蛋白质支架结合剂的产生就是例证。高通量生成亲和试剂的策略也为在全蛋白质组水平上生成特异性蛋白质探针开辟了可能性。最近，这种亲和蛋白质组学使得能够使用组织微阵列和共聚焦显微镜对正常组织和疾病组织中的人类蛋白质表达进行全面的详细分析。
