Lavoie Jean-Claude, Rouleau Thérèse, Tsopmo Apollinaire, Friel James, Chessex Philippe
Department of Paediatrics, CHU Sainte-Justine, University of Montréal, Montréal, Qc, Canada H3T 1C5.
Free Radic Biol Med. 2008 Sep 1;45(5):572-7. doi: 10.1016/j.freeradbiomed.2008.04.018. Epub 2008 Apr 23.
Parenteral multivitamins (MVP) are linked to the generation of peroxides, which cause oxidant injury in lungs associated with alveolar remodelling linked to lung disease of prematurity. This study was to investigate the relationship between alveolar development and lung oxidant-antioxidant status as modulated by the mode of administration of multivitamins with total parenteral nutrition (TPN). Four groups of guinea pig pups received parenteral nutrition differing by 1) mode of MVP admixture: with amino acid solution (AA-MVP) or lipid emulsion (LIP-MVP); 2) light exposure: TPN exposed (LE) or shielded from light (LP). After 2 or 4 days of TPN, vitamins C and E, 8-isoprostaneF2alpha and alveolarization index were determined in lungs and GSSG/GSH in lungs and blood. Exposure to light and the mode of MVP admixture did not influence vitamin E and isoprostane levels. Blood glutathione redox potential was more oxidized in LE and LIP-MVP groups after 4-day infusions, whereas lung redox potential was more reduced in LE groups. LP and LIP-MVP had a beneficial effect, with higher number of alveoli. Globally, results indicate that in this model, alveolarization and modifications in lung redox potential are two independent events induced by light exposed TPN.
肠外多种维生素（MVP）与过氧化物的产生有关，过氧化物会在肺部造成氧化损伤，这种损伤与早产相关肺部疾病所导致的肺泡重塑有关。本研究旨在探讨在全肠外营养（TPN）中多种维生素的给药方式对肺泡发育与肺氧化还原状态之间关系的影响。四组豚鼠幼崽接受不同的肠外营养，区别在于：1）MVP混合方式：与氨基酸溶液混合（AA-MVP）或与脂质乳剂混合（LIP-MVP）；2）光照情况：TPN暴露于光下（LE）或避光（LP）。在进行2天或4天的TPN后，测定肺组织中的维生素C和E、8-异前列腺素F2α以及肺泡化指数，同时测定肺组织和血液中的氧化型谷胱甘肽/还原型谷胱甘肽（GSSG/GSH）。光照和MVP混合方式不影响维生素E和异前列腺素水平。4天输注后，LE组和LIP-MVP组血液中的谷胱甘肽氧化还原电位氧化程度更高，而LE组肺组织中的氧化还原电位还原程度更高。LP组和LIP-MVP组具有有益作用，肺泡数量更多。总体而言，结果表明在此模型中，肺泡化和肺氧化还原电位的改变是光暴露TPN诱导的两个独立事件。
