MacDonnell P C, Tolson N, Guroff G
J Biol Chem. 1977 Aug 25;252(16):5859-63.
Tyrosine hydroxylase is synthesized de novo in rat superior cervical ganglia in organ culture. The differential rate of synthesis is not increased significantly by the addition of nerve growth factor to the culture. Prior administration of nerve growth factor in vivo, however, leads to an augmented synthesis of tyrosine hydroxylase in ganglia subsequently cultured in vitro. The differential rate of tyrosine hydroxylase synthesis was increased by a factor of between 3 and 4. Increases in the differential rate of synthesis were detected within 6 h; the rate reached a maximum 24 to 36 h after a single injection of nerve growth factor. Administration of actinomycin D or of nerve growth factor antibody in vivo prevented the nerve growth factor-induced increase in the differential rate of tyrosine hydroxylase synthesis in vitro. However, the increase in the synthetic rate of tyrosine hydroxylase was not prevented by the addition of actinomycin D to the culture.
酪氨酸羟化酶在器官培养的大鼠颈上神经节中从头合成。向培养物中添加神经生长因子不会显著提高合成差异率。然而，预先在体内给予神经生长因子会导致随后在体外培养的神经节中酪氨酸羟化酶的合成增加。酪氨酸羟化酶合成差异率增加了3至4倍。在6小时内检测到合成差异率增加；单次注射神经生长因子后24至36小时达到最大值。在体内给予放线菌素D或神经生长因子抗体可阻止神经生长因子诱导的体外酪氨酸羟化酶合成差异率增加。然而，向培养物中添加放线菌素D并不能阻止酪氨酸羟化酶合成速率的增加。
