Alves M J, Abuin G, Kuwajima V Y, Colli W
Mol Biochem Parasitol. 1986 Oct;21(1):75-82. doi: 10.1016/0166-6851(86)90081-2.
Monoclonal antibodies were raised against the surface of trypomastigote forms of Trypanosoma cruzi. Although some of these antibodies reacted against antigens shared by trypomastigote and epimastigote or amastigote forms, the majority were trypomastigote-specific. Trypomastigote-specific monoclonal antibodies recognized all infective stages, including trypomastigotes from the bloodstream of infected mice, insect feces, tissue culture and those resulting from differentiation of epimastigotes in axenic culture media. The monoclonal antibodies H1A10 and 6A2, as well as Fab fragments from H1A10, partially prevented T. cruzi invasion of LLC-MK2 cell monolayers (inhibition of 50-70%) when present throughout the entire experiment. Both antibodies recognized an 85 kDa glycoprotein (Tc-85) of the trypomastigote surface which contains N-acetyl-D-glucosamine and/or sialic acid.
制备了针对克氏锥虫锥鞭毛体表面的单克隆抗体。尽管其中一些抗体与锥鞭毛体、上鞭毛体或无鞭毛体形式共有的抗原发生反应，但大多数是锥鞭毛体特异性的。锥鞭毛体特异性单克隆抗体识别所有感染阶段，包括来自感染小鼠血液、昆虫粪便、组织培养物中的锥鞭毛体，以及在无细胞培养基中由上鞭毛体分化产生的锥鞭毛体。单克隆抗体H1A10和6A2以及H1A10的Fab片段在整个实验过程中存在时，部分阻止了克氏锥虫对LLC-MK2细胞单层的侵袭（抑制率为50-70%）。两种抗体都识别锥鞭毛体表面的一种85 kDa糖蛋白（Tc-85），该糖蛋白含有N-乙酰-D-葡萄糖胺和/或唾液酸。
