Wilson H J, Goodman R M, Israel H W
Arch Virol. 1976;51(4):347-54. doi: 10.1007/BF01317938.
Pinwheel inclusions (PWs) were found in cells of callus tissue derived from explants of secondary phloem parenchyma of carrot (Daucus carota) storage root and grown on a basal medium containing zeatin and indoleacetic acid or coconut milk, naphthalene acetic acid, or combinations of these. Preliminary attempts to demonstrate the presence of viruses in the callus tissue failed. The possibility that the tissles were infected by a low titer or unstable conventional virus or by a defective mutant has not been ruled out. However, two lines of evidence suggest that the PWs in these tissues may be a result of culture conditions and not of virus infection. First, no PWs or other cytoplasmic inclusions were found in cells of otherwise similar tissue cultured on basal medium alone, and multifibrillar bundles (MFBs) but not PWs were found when the tissues were cultured on a medium that stimulates differentiation and morphogenesis. Second, culture stimulated to differentiate and containing MFBs only were returned to the supplemented basal medium and subsequently found to contain both PWs and MFBs.
在由胡萝卜（Daucus carota）贮藏根次生韧皮部薄壁组织外植体诱导产生并在含有玉米素和吲哚乙酸或椰乳、萘乙酸或这些物质组合的基础培养基上生长的愈伤组织细胞中发现了风车状内含物（PW）。初步尝试证明愈伤组织中存在病毒，但未成功。这些组织被低滴度或不稳定的传统病毒或有缺陷的突变体感染的可能性尚未排除。然而，有两条证据表明，这些组织中的PW可能是培养条件造成的，而非病毒感染所致。第一，在仅在基础培养基上培养的其他类似组织的细胞中未发现PW或其他细胞质内含物，而当组织在刺激分化和形态发生的培养基上培养时，发现有多纤维束（MFB）但没有PW。第二，仅含有MFB且被刺激分化的培养物被放回添加了成分的基础培养基中，随后发现既含有PW也含有MFB。
