Espinoza A M, Medina V, Hull R, Markham P G
Cellular and Molecular Biology Research Center, University of Costa Rica, San Jose, Central America.
Virology. 1991 Nov;185(1):337-44. doi: 10.1016/0042-6822(91)90781-6.
Turnip leaves infected with the aphid transmissible isolate of cauliflower mosaic virus (CaMV Cabb B-JI) showed two types of virus-containing inclusion bodies (IBs), which differed morphologically and in their protein composition when analyzed by immunogold labeling of ultrathin sections. Vacuolated IBs, typical of CaMV infections, contained P62 (the generally accepted IB protein) but lacked P18 (the aphid transmission factor), while electron-lucent IBs did not contain P62 but were the only detectable sites of P18 accumulation within the infected leaf cells. Both types of inclusions were detected in cells of the epidermis, vascular bundles, mesophyll, and spongy parenchyma. Electron-lucent IBs were not found in the aphid nontransmissible isolates of CaMV, Campbell and CM4-184.
感染了花椰菜花叶病毒蚜虫传播分离株（CaMV Cabb B-JI）的芜菁叶表现出两种含病毒的包涵体（IBs），通过超薄切片免疫金标记分析，它们在形态和蛋白质组成上存在差异。空泡化包涵体是CaMV感染的典型特征，含有P62（普遍认可的包涵体蛋白）但缺乏P18（蚜虫传播因子），而电子透明包涵体不含P62，但却是受感染叶细胞内P18积累的唯一可检测部位。在表皮、维管束、叶肉和海绵薄壁组织的细胞中都检测到了这两种类型的包涵体。在CaMV的蚜虫非传播分离株Campbell和CM4-184中未发现电子透明包涵体。
