Carrasco L, Mena K D, Mota L C, Ortiz M, Behravesh C B, Gibbs S G, Bristol J R, Mayberry L, Cardenas V M
Center for Environmental Resource Management, the University of Texas at El Paso, El Paso, TX 79902, USA.
Lett Appl Microbiol. 2008 Jun;46(6):682-7. doi: 10.1111/j.1472-765X.2008.02378.x. Epub 2008 May 10.
The study aim was to determine the presence of total and faecal coliforms on kitchen surfaces, in tap water and on the hands of caregivers in households on both sides of the US-Mexico border.
Samples were collected in 135 randomly selected households in Ciudad Juarez, Mexico, and El Paso, Texas. Different surfaces throughout the kitchen and head of households' hands were sampled using sterile cotton swabs moistened in D/E neutralizing solution. Sponge/dishcloth and drinking water samples were also obtained. Total and faecal coliforms were enumerated on m-Endo LES and mFC respectively. Total coliforms and Escherichia coli in drinking water samples were enumerated in accordance with the Quanti-Tray method. Sponge/dishcloth samples were the most commonly contaminated kitchen sites, followed by countertops and cutting boards. We recovered faecal coliforms from 14% of the hands of child caregivers, and this indicator was moderately associated with self-reported failure to wash hands after using the toilet (OR = 3.2; 95% CI: 0.9, 11.1).
Hand washing should continue to be emphasized, and additional interventions should be directed to specific kitchen areas, such as sponges/dishcloths, tables/countertops and cutting boards.
There is a need for additional interventions regarding kitchen sanitation.
本研究旨在确定美墨边境两侧家庭厨房表面、自来水及护理人员手上的总大肠菌群和粪大肠菌群的存在情况。
在墨西哥华雷斯城和得克萨斯州埃尔帕索随机选取135户家庭采集样本。使用浸有D/E中和溶液的无菌棉签对厨房各处表面及户主的手部进行采样。还获取了海绵/洗碗布和饮用水样本。分别在m - 远藤亮绿胆盐（m-Endo LES）和m - 发酵乳糖培养基（mFC）上对总大肠菌群和粪大肠菌群进行计数。饮用水样本中的总大肠菌群和大肠杆菌按照定量盘法进行计数。海绵/洗碗布样本是厨房中最常被污染的部位，其次是台面和砧板。我们从14%的儿童护理人员手上检测出粪大肠菌群，该指标与自我报告的便后未洗手情况中度相关（比值比 = 3.2；95%置信区间：0.9，11.1）。
应继续强调洗手，并应对特定的厨房区域，如海绵/洗碗布、桌子/台面和砧板采取额外干预措施。
需要针对厨房卫生采取更多干预措施。
