用于真菌毒素检测的纳米结构氧化锌平台。
Nanostructured zinc oxide platform for mycotoxin detection.
机构信息
Department of Science & Technology Centre on Biomolecular Electronics, New Delhi, India.
出版信息
Bioelectrochemistry. 2010 Feb;77(2):75-81. doi: 10.1016/j.bioelechem.2009.06.014. Epub 2009 Jul 8.
Nanostructured zinc oxide (Nano-ZnO) film has been deposited onto indium-tin-oxide (ITO) glass plate for co-immobilization of rabbit-immunoglubin antibodies (r-IgGs) and bovine serum albumin (BSA) for ochratoxin-A (OTA) detection. The results of X-ray diffraction (XRD) studies reveal the formation of Nano-ZnO with average particle size as ~5.0nm. Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) techniques have been used to characterize Nano-ZnO/ITO electrode and BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode. Electrochemical impedimetric response of BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode obtained as a function of OTA concentration exhibits linearity as 0.006-0.01nM/dm(3), detection limit of 0.006nM/dm(3), response time as 25s and sensitivity of 189Omega/nM/dm(3)cm(-2) with a regression coefficient of 0.997.
纳米结构氧化锌（Nano-ZnO）薄膜沉积在氧化铟锡（ITO）玻璃上，用于共固定兔免疫球蛋白抗体（r-IgGs）和牛血清白蛋白（BSA），以检测赭曲霉毒素 A（OTA）。X 射线衍射（XRD）研究结果表明，形成了平均粒径约为~5.0nm 的 Nano-ZnO。傅里叶变换红外（FTIR）光谱、扫描电子显微镜（SEM）、电化学阻抗谱（EIS）技术用于表征 Nano-ZnO/ITO 电极和 BSA/r-IgGs/Nano-ZnO/ITO 免疫电极。作为 OTA 浓度的函数获得的 BSA/r-IgGs/Nano-ZnO/ITO 免疫电极的电化学阻抗响应表现出线性关系，线性范围为 0.006-0.01nM/dm(3)，检测限为 0.006nM/dm(3)，响应时间为 25s，灵敏度为 189Omega/nM/dm(3)cm(-2)，回归系数为 0.997。
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