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从丹麦禽类中的后院家禽和两次禽霍乱爆发中获得的多杀巴斯德氏菌分离株的表型和基因型特征。

Phenotypic and genotypic characters of isolates of Pasteurella multocida obtained from back-yard poultry and from two outbreaks of avian cholera in avifauna in Denmark.

机构信息

Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.

出版信息

Avian Pathol. 1998;27(4):373-81. doi: 10.1080/03079459808419354.

Abstract

Two outbreaks of fowl cholera in the avifauna in Denmark, affecting primarily eiders but also cormorants, gulls and oyster-catchers were shown to be caused by the same clone of Pasteurella multocida ssp. multocida by restriction enzyme analysis (REA) and ribotyping, using the enzymes HpaII and HhaI and phenotypic characterization. This observation indicated spread by migratory birds. It was shown that the outbreak clone was closely related to isolates of Pasteurella multocida ssp. multocida obtained from back-yard poultry in Denmark, including chickens, pheasants, turkeys and ducks. The only detectable difference between the outbreak clone and some of these strains concerned the size of one fragment. These results indicate a possible exchange of P. multocida ssp. multocida between populations of wild birds and back-yard poultry. Among the DNA fingerprinting methods used, restriction enzyme analysis offered the highest discrimination among thirty strains obtained from back-yard poultry. The restriction enzymes HpaII and HhaI generated almost the same number of profile types, 17 and 15 respectively, but only HpaII differentiated the outbreak clone from the group of closely related strains isolated from back-yard poultry. Ribotyping, using the same enzymes, resulted in 12 and 10 different profile types, respectively. The outbreak isolates did not harbour any plasmids, while six out of the 30 strains originating from back-yard poultry (20%) carried a cryptic plasmid of approximately 3.4 kb.

摘要

丹麦水禽中暴发的两次禽霍乱,主要影响绒鸭,但也影响鸬鹚、海鸥和蛎鹬,通过限制酶分析(REA)和核糖体分型,使用 HpaII 和 HhaI 酶和表型特征,证明这些疫情是由同一克隆巴氏杆菌引起的。这一观察结果表明,疫情是由候鸟传播的。研究表明,暴发的克隆与从丹麦后院家禽(包括鸡、野鸡、火鸡和鸭)中获得的多杀巴斯德氏菌亚种多杀巴斯德氏菌的分离株密切相关。暴发克隆与其中一些菌株之间唯一可检测到的差异涉及一个片段的大小。这些结果表明,野生鸟类和后院家禽种群之间可能存在多杀巴斯德氏菌亚种多杀巴斯德氏菌的交换。在所使用的 DNA 指纹图谱方法中,限制酶分析在从后院家禽获得的 30 株菌株中提供了最高的分辨率。限制酶 HpaII 和 HhaI 分别产生了 17 和 15 种不同的图谱类型,但只有 HpaII 能将暴发的克隆与从后院家禽中分离出的一组密切相关的菌株区分开来。使用相同的酶进行核糖体分型,分别产生了 12 和 10 种不同的图谱类型。暴发分离株不携带任何质粒,而从后院家禽中分离出的 30 株菌株中有 6 株(20%)携带一个约 3.4kb 的隐性质粒。

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