Mesia-Vela Sonia, Yeh Chih-Ching, Austin John H M, Dounel Matthew, Powell Charles A, Reeves Anthony, Santella Regina M, Stevenson Lori, Yankelevitz David, Barr R Graham
Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
Biomarkers. 2008 Jun;13(4):422-34. doi: 10.1080/13547500802002859.
Oxidative stress and inflammation are hallmarks of chronic obstructive pulmonary disease (COPD). A critical byproduct of oxidative damage is the introduction of carbonyl groups into amino acid residues. We hypothesize that plasma carbonyl content is inversely correlated with lung function and computed tomography (CT) measures of lung density among smokers and is elevated in COPD. Carbonyl was measured in plasma of participants aged 60 years and older by ELISA. Generalized linear and additive models were used to adjust for potential confounders. Among 541 participants (52% male, mean age 67 years, 41% current smokers), mean plasma carbonyl content was 17.9+/-2.9 nmol ml(-1) and mean forced expiratory volume in one second (FEV(1)) was 80.7+/-20.9% of predicted. Plasma carbonyl content was inversely associated with FEV(1), but this relationship was largely explained by age. Multivariate analyses ruled out clinically meaningful associations of plasma carbonyl content with FEV(1), FEV(1)/FVC (forced vital capacity) ratio, severity of airflow obstruction, and CT lung density. Plasma carbonyl content is a poor biomarker of oxidative stress in COPD and emphysema.
氧化应激和炎症是慢性阻塞性肺疾病（COPD）的标志。氧化损伤的一个关键副产物是将羰基引入氨基酸残基。我们假设，在吸烟者中，血浆羰基含量与肺功能和肺部密度的计算机断层扫描（CT）测量值呈负相关，且在COPD患者中升高。通过酶联免疫吸附测定法（ELISA）测量了60岁及以上参与者血浆中的羰基含量。使用广义线性模型和加法模型对潜在混杂因素进行校正。在541名参与者中（52%为男性，平均年龄67岁，41%为当前吸烟者），血浆羰基含量的平均值为17.9±2.9 nmol/ml（-1），一秒用力呼气量（FEV₁）的平均值为预测值的80.7±20.9%。血浆羰基含量与FEV₁呈负相关，但这种关系在很大程度上可由年龄来解释。多变量分析排除了血浆羰基含量与FEV₁、FEV₁/FVC（用力肺活量）比值、气流阻塞严重程度及CT肺密度之间具有临床意义的关联。血浆羰基含量是COPD和肺气肿中氧化应激的一个不良生物标志物。
