Liu Mei, Dai Yue, Li Ying, Luo Yubin, Huang Fang, Gong Zhunan, Meng Qingyu
Department of Pharmacology of China Materia Medica, China Pharmaceutical University, Nanjing, People's Republic of China.
Planta Med. 2008 Jun;74(8):809-15. doi: 10.1055/s-2008-1074533. Epub 2008 May 16.
The current study was designed to investigate the effect of madecassoside, the major triterpene in CENTELLA ASIATICA, on burn wound healing and its possible mechanism of action. An oral administration of madecassoside (6, 12, 24 mg/kg) facilitated wound closure in a time-dependent manner and reached its peak effect, nearly completely wound closure, on day 20 in the group receiving the highest dose of 24 mg/kg of madecassoside. Further histopathological analysis revealed that madecassoside alleviated infiltration of inflammatory cells as well as enhanced epithelisation resulting from dermal proliferation of fibroblasts. Madecassoside at higher doses (12 and 24 mg/kg) decreased nitric oxide (NO) levels and malondialdehyde (MDA) content in the burn skin tissue. However, reduced glutathione (GSH) and hydroxyproline levels were increased in the same skin tissue. In addition, madecassoside promoted skin angiogenesis IN VIVO, correlating with our findings IN VITRO that it stimulated endothelial cell growth in a rat aortic ring assay. These data suggest that madecassoside has significant wound-healing activity and is one of the major reasons for the use of C. ASIATICA herbs in the successful treatment of burn injury. Moreover, the results from the present study indicate that the effect of madecassoside on wound healing may involve several mechanisms including antioxidative activity, collagen synthesis and angiogenesis.
本研究旨在探讨积雪草中的主要三萜成分积雪草苷对烧伤创面愈合的影响及其可能的作用机制。口服积雪草苷（6、12、24mg/kg）能以时间依赖性方式促进创面愈合，在接受最高剂量24mg/kg积雪草苷的组中，于第20天达到峰值效应，创面几乎完全愈合。进一步的组织病理学分析显示，积雪草苷减轻了炎症细胞浸润，并增强了由成纤维细胞真皮增殖导致的上皮形成。较高剂量（12和24mg/kg）的积雪草苷降低了烧伤皮肤组织中的一氧化氮（NO）水平和丙二醛（MDA）含量。然而，同一皮肤组织中的还原型谷胱甘肽（GSH）和羟脯氨酸水平有所升高。此外，积雪草苷在体内促进了皮肤血管生成，这与我们在体外大鼠主动脉环试验中发现其刺激内皮细胞生长的结果相关。这些数据表明，积雪草苷具有显著的创面愈合活性，是积雪草成功治疗烧伤的主要原因之一。此外，本研究结果表明，积雪草苷对创面愈合的作用可能涉及多种机制，包括抗氧化活性、胶原蛋白合成和血管生成。
