Gao Qiu T, Cheung Jerry K, Choi Roy C, Cheung Anna W, Li Jun, Jiang Zhi Y, Duan Ran, Zhao Kui J, Ding An W, Dong Tina T, Tsim Karl W
Department of Biology and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, P. R. China.
Planta Med. 2008 Mar;74(4):392-5. doi: 10.1055/s-2008-1034322.
Danggui Buxue Tang (DBT), a Chinese medicinal decoction used commonly for treating women's ailments, contains Radix Astragali (RA) and Radix Angelicae Sinensis (RAS). According to Chinese medicinal theory, this decoction is to nourish the blood function; this, however, has not been demonstrated on the molecular level. In order to reveal the hematopoietic effect of this decoction, DBT was applied to cultured Hep3B human hepatocellular carcinoma cells. The treatment of DBT induced mRNA expression of erythropoietin (EPO) in a dose-dependent manner and peaked at approximately 2.5-fold induction. The secreted EPO in cultured Hep3B cells was quantified by ELISA: the treatment of DBT potentiated the effect of hypoxia-induced EPO expression in the cultured cells. In addition, the DBT-induced EPO expression could be abolished by pre-treatment with U0126, a mitogen-activated kinase inhibitor. The current results verified the hematopoietic function of this ancient herbal decoction.
当归补血汤（DBT）是一种常用于治疗女性疾病的中药汤剂，由黄芪（RA）和当归（RAS）组成。根据中医理论，该汤剂具有补血功效；然而，这尚未在分子水平上得到证实。为了揭示该汤剂的造血作用，将DBT应用于培养的Hep3B人肝癌细胞。DBT处理以剂量依赖方式诱导促红细胞生成素（EPO）的mRNA表达，诱导倍数约为2.5倍时达到峰值。通过酶联免疫吸附测定（ELISA）对培养的Hep3B细胞中分泌的EPO进行定量：DBT处理增强了缺氧诱导培养细胞中EPO表达的作用。此外，用丝裂原活化激酶抑制剂U01A26预处理可消除DBT诱导的EPO表达。目前的结果证实了这种古老中药汤剂的造血功能。
