Wakusawa Ryosuke, Abe Toshiaki, Sato Hajime, Yoshida Madoka, Kunikata Hiroshi, Sato Yasufumi, Nishida Kohji
Department of Ophthalmology and Visual Science, Tohoku University Graduate School of Medicine, Miyagi, Japan.
Am J Ophthalmol. 2008 Aug;146(2):235-243. doi: 10.1016/j.ajo.2008.03.019. Epub 2008 May 16.
To determine whether vasohibin, an antiangiogenic factor produced by vascular endothelial cells, is expressed in the choroidal neovascular (CNV) membranes obtained from human eyes with age-related macular degeneration (AMD) or polypoidal choroidal vasculopathy (PCV).
Retrospective, interventional case series.
The medical charts of 21 eyes of 21 patients with AMD or PCV who underwent surgical removal of the CNV membrane were reviewed. The removed tissues were immunostained for von Willebrand Factor (vWF), vascular endothelial growth factor (VEGF), and vasohibin. The levels of the messenger ribonucleic acid of VEGF, VEGFR2, and vasohibin were determined by real-time reverse-transcriptase polymerase chain reaction (RT-PCR) from the CNV membranes excised from nine AMD and nine PCV patients.
The patients were divided into three groups; four patients were placed in the most active group (Group H), 13 in the less active group (Group E), and four in the nonactive group (Group S). Immunohistochemistry showed that vasohibin, vWF, and VEGF were expressed in the vascular endothelial cells in the CNV membranes and in the polypoidal vessels. RT-PCR showed that there was a strong correlation between the level of expression of VEGFR2 and vasohibin (P = .0002). Eyes with a lower vasohibin-to-VEGF ratio tended to have larger subretinal hemorrhages or vitreous hemorrhages, whereas eyes with higher vasohibin-to-VEGF ratio had subretinal fibrosislike lesions. Statistical analysis of the vasohibin-to-VEGF ratio among the three groups was significant (P = .0209).
Vasohibin is expressed in human CNV membranes. Our results indicate that the vasohibin-to-VEGF ratio may be related with the activity of the CNV.
确定血管内皮细胞产生的抗血管生成因子血管抑制素是否在年龄相关性黄斑变性（AMD）或息肉状脉络膜血管病变（PCV）患者的人眼脉络膜新生血管（CNV）膜中表达。
回顾性、介入性病例系列研究。
回顾了21例接受CNV膜手术切除的AMD或PCV患者的21只眼的病历。对切除的组织进行免疫染色，检测血管性血友病因子（vWF）、血管内皮生长因子（VEGF）和血管抑制素。通过实时逆转录聚合酶链反应（RT-PCR）测定从9例AMD和9例PCV患者切除的CNV膜中VEGF、VEGFR2和血管抑制素的信使核糖核酸水平。
患者分为三组；4例患者归入最活跃组（H组），13例归入较不活跃组（E组），4例归入非活跃组（S组）。免疫组织化学显示，血管抑制素、vWF和VEGF在CNV膜的血管内皮细胞和息肉状血管中表达。RT-PCR显示，VEGFR2和血管抑制素的表达水平之间存在强相关性（P = 0 . 0002）。血管抑制素与VEGF比值较低的眼往往有较大的视网膜下出血或玻璃体积血，而血管抑制素与VEGF比值较高的眼有视网膜下纤维化样病变。三组间血管抑制素与VEGF比值的统计学分析具有显著性（P = 0 . 0209）。
血管抑制素在人CNV膜中表达。我们的结果表明，血管抑制素与VEGF的比值可能与CNV的活性有关。
