Taketo M, Schroeder A C, Mobraaten L E, Gunning K B, Hanten G, Fox R R, Roderick T H, Stewart C L, Lilly F, Hansen C T
Jackson Laboratory, Bar Harbor, ME 04609.
Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2065-9. doi: 10.1073/pnas.88.6.2065.
FVB/N mice offer a system suitable for most transgenic experiments and subsequent genetic analyses. The inbred FVB/N strain is characterized by vigorous reproductive performance and consistently large litters. Moreover, fertilized FVB/N eggs contain large and prominent pronuclei, which facilitate microinjection of DNA. The phenotype of large pronuclei in the zygote is a dominant trait associated with the FVB/N oocyte but not the FVB/N sperm. In experiments to generate transgenic mice, the same DNA constructs were injected into three different types of zygotes: FVB/N, C57BL/6J, and (C57BL/6J x SJL/J)F1. FVB/N zygotes survived well after injection, and transgenic animals were obtained with efficiencies similar to the F1 zygotes and much better than the C57BL/6J zygotes. Genetic markers of the FVB/N strain have been analyzed for 44 loci that cover 15 chromosomes and were compared with those of commonly used inbred strains. In addition to the albino FVB/N strain, pigmented congenic strains of FVB/N are being constructed. These features make the FVB/N strain advantageous to use for research with transgenic mice.
FVB/N小鼠提供了一个适用于大多数转基因实验及后续遗传分析的系统。近交FVB/N品系的特点是繁殖性能旺盛且产仔数始终较多。此外，受精的FVB/N卵含有大而明显的原核，这便于DNA的显微注射。合子中存在大原核这一表型是与FVB/N卵母细胞相关的显性性状，而非FVB/N精子。在生成转基因小鼠的实验中，将相同的DNA构建体注射到三种不同类型的合子中：FVB/N、C57BL/6J和（C57BL/6J×SJL/J）F1。FVB/N合子注射后存活良好，获得转基因动物的效率与F1合子相似，且远高于C57BL/6J合子。已对FVB/N品系的44个覆盖15条染色体的基因座进行了遗传标记分析，并与常用近交品系的标记进行了比较。除了白化FVB/N品系外，还正在构建FVB/N的有色同源品系。这些特性使得FVB/N品系有利于用于转基因小鼠的研究。
