Wu Zhen-Xing, Wang Hui-Zhong, Shi Nong-Nong, Zhao Yan
Key Laboratory of Biochemistry and Molecular Biology, Hangzhou Normal University, Hangzhou 310018,China.
Yi Chuan. 2008 May;30(5):627-32. doi: 10.3724/sp.j.1005.2008.00627.
ISSR was applied to detect the relationship between 16 Cymbidium species, and 836 bands were amplified with 15 primers, including 227 polymorphic bands. The polymorphic percentage is 27.2%. UPGMA results showed that the genetic distance were closest between C.goeringii (Rchb.f.) Rchb.f. and C. goeringii var. longibracteatum, and C.lancifolium Hook. was far away from the other 15 species. This result is quite similar to the traditional classification, indicating that the technique could supplement some information to traditional taxonomy in the molecular level.
利用ISSR技术检测16种兰花的亲缘关系，15条引物共扩增出836条带，其中多态性条带227条，多态性百分比为27.2%。UPGMA结果显示，春兰与长苞春兰之间的遗传距离最近，而建兰与其他15个种的遗传距离较远。这一结果与传统分类较为相似，表明该技术可在分子水平上为传统分类学补充一些信息。
