鼻疽伯克霍尔德菌的BmaR3-BmaI3群体感应系统产生并响应N-3-羟基辛酰高丝氨酸内酯。
The Burkholderia mallei BmaR3-BmaI3 quorum-sensing system produces and responds to N-3-hydroxy-octanoyl homoserine lactone.
作者信息
Duerkop Breck A, Herman Jake P, Ulrich Ricky L, Churchill Mair E A, Greenberg E Peter
机构信息
Department of Microbiology, University of Washington School of Medicine, Seattle, Washington 98195-7242, USA.
出版信息
J Bacteriol. 2008 Jul;190(14):5137-41. doi: 10.1128/JB.00246-08. Epub 2008 May 16.
Abstract
Burkholderia mallei has two acyl-homoserine lactone (acyl-HSL) signal generator-receptor pairs and two additional signal receptors, all of which contribute to virulence. We show that B. mallei produces N-3-hydroxy-octanoyl HSL (3OHC8-HSL) but a bmaI3 mutant does not. Recombinant Escherichia coli expressing BmaI3 produces hydroxylated acyl-HSLs, with 3OHC8-HSL being the most abundant compound. In recombinant E. coli, BmaR3 responds to 3OHC8-HSL but not to other acyl-HSLs. These data indicate that the signal for BmaR3-BmaI3 quorum sensing is 3OHC8-HSL.
摘要
鼻疽伯克霍尔德菌有两对酰基高丝氨酸内酯（acyl-HSL）信号生成-受体对以及另外两个信号受体，所有这些都与毒力有关。我们发现鼻疽伯克霍尔德菌产生N-3-羟基辛酰高丝氨酸内酯（3OHC8-HSL），但bmaI3突变体不产生。表达BmaI3的重组大肠杆菌产生羟基化的酰基高丝氨酸内酯，其中3OHC8-HSL是最丰富的化合物。在重组大肠杆菌中，BmaR3对3OHC8-HSL有反应，但对其他酰基高丝氨酸内酯没有反应。这些数据表明，BmaR3-BmaI3群体感应的信号是3OHC8-HSL。
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