López-Expósito I, Pellegrini A, Amigo L, Recio I
Instituto de Fermentaciones Industriales, Consejo Superior de Investigaciones Científicas, Juan de la Cierva 3, 28006 Madrid, Spain.
J Dairy Sci. 2008 Jun;91(6):2184-9. doi: 10.3168/jds.2007-0037.
Antimicrobial peptides derived from food proteins constitute a new field in the combined use of antimicrobial agents in food. The best examples of milk-derived peptides are those constituted by bovine lactoferricin [lactoferrin f(17-41)] (LFcin-B) and bovine alpha(s2)-casein f(183-207). The aim of this work was to study if the antimicrobial activity of a natural compound employed in food preservation, nisin, could be enhanced by combination with the aforementioned milk-derived peptides. Furthermore, the possibility of a synergistic effect between these peptides and bovine lactoferrin (LF) against Escherichia coli and Staphylococcus epidermidis was also studied. Finally, the most active combinations were assayed against the foodborne pathogens Listeria monocytogenes and Salmonella choleraesuis. Results showed a synergistic effect when LFcin-B was combined with bovine LF against E. coli. In the same way, the combination of LFcin-B with bovine LF was synergistic against Staph. epidermidis. Bovine LF and nisin increased their antimicrobial activity when they were assayed together with bovine alpha(s2)-casein f(183-207). It is important to note the synergistic effect among LFcin-B and bovine LF, because both compounds might be simultaneously in the suckling gastrointestinal tract and could, therefore, have a protective effect on it. The other synergistic effect high-lighted is that between alpha(s2)-casein f(183-207) and nisin against L. monocytogenes because of the ability of L. monocytogenes to develop resistance to nisin.
源自食物蛋白的抗菌肽是食品中抗菌剂联合使用的一个新领域。源自牛奶的肽的最佳例子是由牛乳铁蛋白[乳铁蛋白f(17 - 41)]（LFcin - B）和牛α(s2)-酪蛋白f(183 - 207)构成的那些肽。这项工作的目的是研究用于食品保鲜的天然化合物乳链菌肽与上述源自牛奶的肽联合使用时，其抗菌活性是否能够增强。此外，还研究了这些肽与牛乳铁蛋白（LF）之间对大肠杆菌和表皮葡萄球菌产生协同效应的可能性。最后，对最具活性的组合针对食源性病原体单核细胞增生李斯特菌和猪霍乱沙门氏菌进行了检测。结果表明，LFcin - B与牛LF联合使用时对大肠杆菌有协同效应。同样，LFcin - B与牛LF的组合对表皮葡萄球菌也有协同作用。牛LF和乳链菌肽与牛α(s2)-酪蛋白f(183 - 207)一起检测时，它们的抗菌活性增强。需要注意LFcin - B与牛LF之间的协同效应，因为这两种化合物可能同时存在于哺乳幼崽的胃肠道中，因此可能对其有保护作用。另一个突出的协同效应是α(s2)-酪蛋白f(183 - 207)与乳链菌肽对单核细胞增生李斯特菌的协同效应，这是由于单核细胞增生李斯特菌有产生对乳链菌肽耐药性的能力。
