新型肽 GQLGEHGGAGMG、GEHGGAGMGGGQFQPV、EQGFLPGPEESGR、RLARAGLAQ、YGNPVGGVGH 和 GNPVGGVGHGTTGT 作为代谢综合征相关酶抑制剂和抗菌潜力的特性。
Characteristics of New Peptides GQLGEHGGAGMG, GEHGGAGMGGGQFQPV, EQGFLPGPEESGR, RLARAGLAQ, YGNPVGGVGH, and GNPVGGVGHGTTGT as Inhibitors of Enzymes Involved in Metabolic Syndrome and Antimicrobial Potential.
机构信息
Department of Biochemistry and Food Chemistry, University of Life Sciences in Lublin, Skromna 8, 20-704 Lublin, Poland.
Department of Environmental Microbiology, University of Life Sciences in Lublin, St. Leszczyńskiego 7, 20-069 Lublin, Poland.
出版信息
Molecules. 2020 May 27;25(11):2492. doi: 10.3390/molecules25112492.
The aim of this study was to determine the cytotoxic properties, influence on enzyme activity involved in metabolic syndrome, and antimicrobial activity of synthetic peptides with GQLGEHGGAGMG, GEHGGAGMGGGQFQPV, EQGFLPGPEESGR, RLARAGLAQ, YGNPVGGVGH, and GNPVGGVGHGTTGT sequences. Peptides have no cytotoxic effect on cells. The highest inhibitory effect on angiotensin converting enzyme I was noted for peptide GT-14 (IC = 525.63 µg/mL). None of the tested peptides had an influence on α-glucosidase. The highest α-amylase and lipase inhibitory activity was noted for GG-12 (IC = 56.72 and 60.62 µg/mL, respectively). The highest lipoxidase inhibitory activity was determined for peptide ER-13 (IC = 84.35 µg/mL). Peptide RQ-9 was characterized by the highest COX inhibitory activity (0.31 and 4.77 µg/mL for COX-1 and COX-2, respectively). Only peptide RQ-9 inhibited ATCC 4931 growth (42%-48%) in all tested concentrations (15.62-250 mg/mL).
本研究旨在确定具有 GQLGEHGGAGMG、GEHGGAGMGGGQFQPV、EQGFLPGPEESGR、RLARAGLAQ、YGNPVGGVGH 和 GNPVGGVGHGTTGT 序列的合成肽的细胞毒性、对代谢综合征相关酶活性的影响和抗菌活性。这些肽对细胞没有细胞毒性作用。肽 GT-14（IC = 525.63 µg/mL）对血管紧张素转化酶 I 的抑制作用最强。测试的肽均未影响α-葡萄糖苷酶。GG-12 对α-淀粉酶和脂肪酶的抑制活性最高（IC = 56.72 和 60.62 µg/mL）。肽 ER-13 的脂氧合酶抑制活性最高（IC = 84.35 µg/mL）。肽 RQ-9 对 COX-1 和 COX-2 的抑制活性最高（分别为 0.31 和 4.77 µg/mL）。只有肽 RQ-9 在所有测试浓度（15.62-250 mg/mL）下均抑制 ATCC 4931 的生长（42%-48%）。
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