Liu Xiao-Cui, Zhu Zhen-Yuan, Tang Ya-Li, Wang Ming-fei, Wang Zheng, Liu An-Jun, Zhang Yong-Min
Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China; Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center, 300457 Tianjin, PR China.
Carbohydr Polym. 2016 May 20;142:63-72. doi: 10.1016/j.carbpol.2016.01.040. Epub 2016 Jan 21.
The structural properties of polysaccharides, respectively, obtained from the fermented mycelium and cultivated fruiting bodies of the Cordyceps militaris were investigated and compared in this paper. First, the crude polysaccharides were extracted from the mycelium and the fruiting bodies, respectively. The polysaccharides were successively purified by Sevag and chromatography on Sephadex G-100 column to produce two polysaccharides fractions termed CMPS-II and CBPS-II, respectively. The average molecular weights of CMPS-II and CBPS-II were 1.402×10(3) kDa and 1.273×10(3) kDa, respectively, and they were mainly composed of mannose, glucose and galactose in the mole ratios of 1:28.63:1.41 and 1:12.41:0.74, respectively, for CMPS-II and CBPS-II. Afterward, the structural features of CMPS-II and CBPS-II were investigated by a combination of chemical and instrumental analysis, such as FT-IR, periodate oxidation-Smith degradation, GC-MS, NMR and methylation analysis. The results indicated that structurally, both CMPS-II and CBPS-II were 1,3-branched-galactomannoglucan that had a linear backbone of (1→4)-linked α-D-glucopyranose (Glcp). Congo-red test revealed that CMPS-II and CBPS-II existed as triple-helical chains in 0.05-0.15 M NaOH solution.
本文对分别从蛹虫草发酵菌丝体和栽培子实体中获得的多糖的结构性质进行了研究和比较。首先，分别从菌丝体和子实体中提取粗多糖。多糖先后通过Sevag法和Sephadex G - 100柱色谱法进行纯化，分别得到两个多糖级分，命名为CMPS - II和CBPS - II。CMPS - II和CBPS - II的平均分子量分别为1.402×10(3) kDa和1.273×10(3) kDa，它们主要由甘露糖、葡萄糖和半乳糖组成，CMPS - II中三者的摩尔比为1:28.63:1.41，CBPS - II中三者的摩尔比为1:12.41:0.74。随后，通过傅里叶变换红外光谱（FT - IR）、高碘酸盐氧化 - 史密斯降解、气相色谱 - 质谱联用（GC - MS）、核磁共振（NMR）和甲基化分析等化学和仪器分析方法相结合，对CMPS - II和CBPS - II的结构特征进行了研究。结果表明，在结构上，CMPS - II和CBPS - II均为1,3 - 分支 - 半乳甘露葡聚糖，其线性主链由(1→4)连接的α - D - 吡喃葡萄糖（Glcp）组成。刚果红试验表明，CMPS - II和CBPS - II在0.05 - 0.15 M NaOH溶液中以三螺旋链形式存在。
