School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Physical Processing of Agricultural Products Key Lab of Jiangsu Province, Zhenjiang 212013, Jiangsu, China.
Carbohydr Polym. 2014 Nov 26;113:380-7. doi: 10.1016/j.carbpol.2014.07.027. Epub 2014 Jul 21.
In this study, a novel flat-plate ultrasound technology was developed to stimulate polysaccharides production from Phellinus igniarius mycelial fermentation. Three-factor-three-level Box-Behnken design was used to optimize ultrasonic treatment time, duty cycle time and culture time for a high yield of P. igniarius polysaccharides (PIPS). Optimal conditions were found to be ultrasound treatment time 65 min, duty cycle time 25s, and culture time 3.8d that gave a maximum PIPS yield of 1.8002 g/L, which increased ∼ 22.64% compared with the control (without any ultrasound). PIPS mainly contained low-molecular weight (MW) polysaccharides (3.1 kDa, 80%) composed of glucose, rhamnose and mannose in a molar ratio of 11.0:14.0:1.0. PIPS with higher carbohydrate and uronic acid contents exhibited strong antioxidant activities in vitro. Laser scanning confocal microscope (LSCM) observations suggested that ultrasound could change the morphology and structure of P. igniarius mycelium, and accelerate the transfer of nutrients and metabolites.
在这项研究中，开发了一种新型的平板超声技术，以刺激火木层孔菌菌丝发酵生产多糖。采用三因素三水平 Box-Behnken 设计对超声处理时间、占空比时间和培养时间进行优化，以获得高产火木层孔菌多糖（PIPS）。最优条件为超声处理时间 65min、占空比时间 25s、培养时间 3.8d，在此条件下，PIPS 的最大产量为 1.8002g/L，比对照组（无任何超声处理）提高了约 22.64%。PIPS 主要含有低分子量（MW）多糖（3.1kDa，80%），由葡萄糖、鼠李糖和甘露糖以摩尔比 11.0:14.0:1.0 组成。具有较高碳水化合物和糖醛酸含量的 PIPS 表现出较强的体外抗氧化活性。激光共聚焦显微镜（LSCM）观察表明，超声可以改变火木层孔菌菌丝的形态和结构，加速营养物质和代谢物的传递。
